BRENDA - Enzyme Database show
show all sequences of 1.1.1.391

Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids

Marschall, H.U.; Oppermann, U.C.; Svensson, S.; Nordling, E.; Persson, B.; Hoeoeg, J.O.; Joernvall, H.; Hepatology 31, 990-996 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant class I ADH g2g2 isozymes cloning and overexpression in Escherichia coli
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0153
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.0183
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.0238
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.0283
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
Homo sapiens
substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant class I ADH g2g2 isozymes from Escherichia coli by anion exchange and 5'-AMP-affinity chromatography
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isochenodeoxycholic acid
736216
Homo sapiens
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate
736216
Homo sapiens
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isoursodeoxycholic acid
736216
Homo sapiens
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta-hydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isolithocholic acid
736216
Homo sapiens
3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isodeoxycholic acid
736216
Homo sapiens
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
additional information
substrate specificity, bile acid screening, reaction product identification and quantification by gas chromotography/mass spectrometry, overview. No activity with 3alpha,7alpha,12alpha,27-tetrahydroxy-5beta-cholestane
736216
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.015
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.022
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.033
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.047
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Homo sapiens
NAD+
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant class I ADH g2g2 isozymes cloning and overexpression in Escherichia coli
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Homo sapiens
NAD+
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0153
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.0183
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.0238
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.0283
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
Homo sapiens
substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant class I ADH g2g2 isozymes from Escherichia coli by anion exchange and 5'-AMP-affinity chromatography
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isochenodeoxycholic acid
736216
Homo sapiens
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate
736216
Homo sapiens
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isoursodeoxycholic acid
736216
Homo sapiens
7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta-hydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isolithocholic acid
736216
Homo sapiens
3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
3beta12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
i.e. isodeoxycholic acid
736216
Homo sapiens
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
?
additional information
substrate specificity, bile acid screening, reaction product identification and quantification by gas chromotography/mass spectrometry, overview. No activity with 3alpha,7alpha,12alpha,27-tetrahydroxy-5beta-cholestane
736216
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.015
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.022
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.033
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
0.047
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
metabolism
epimerization of the 3beta-hydroxy to a 3alpha-hydroxy group of epi-bile acids catalyzed by 3beta-hydroxysteroid dehydrogenases and 3-oxo-reductases with 3-dehydro (oxo) bile acids as intermediate products
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
epimerization of the 3beta-hydroxy to a 3alpha-hydroxy group of epi-bile acids catalyzed by 3beta-hydroxysteroid dehydrogenases and 3-oxo-reductases with 3-dehydro (oxo) bile acids as intermediate products
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.82
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
1.4
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
1.4
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
1.66
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.82
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
1.4
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
1.4
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
1.66
-
3beta-hydroxy-5beta-cholan-24-oic acid
pH 7.5, 25°C, recombinant enzyme
Homo sapiens
Other publictions for EC 1.1.1.391
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736859
Devlin
A biosynthetic pathway for a p ...
Eggerthella lenta, Eggerthella lenta ATCC 25559
Nat. Chem. Biol.
11
685-690
2015
-
-
1
-
-
-
-
2
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
2
1
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
4
-
2
-
-
2
2
-
-
-
-
2
4
-
-
-
736216
Marschall
Human liver class I alcohol de ...
Homo sapiens
Hepatology
31
990-996
2000
-
-
1
-
-
-
-
4
1
-
-
1
-
2
-
-
1
-
-
1
-
-
6
-
1
-
-
4
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
1
-
-
1
-
-
-
1
-
1
-
-
6
-
1
-
-
4
1
-
-
-
-
1
1
-
4
4
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta, Blautia producta K2/25-1
Biochim. Biophys. Acta
1004
230-238
1989
-
-
-
-
-
1
1
-
1
-
1
-
-
2
-
-
1
-
-
-
8
-
6
-
1
-
3
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
1
-
-
-
-
1
-
-
8
-
6
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
735471
Edenharder
Partial purification and chara ...
Clostridium innocuum, Clostridium innocuum D1144/3
Appl. Environ. Microbiol.
55
1656-1659
1989
-
-
-
-
-
1
1
1
1
-
1
4
-
2
-
-
1
-
-
1
1
1
11
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
1
1
-
1
4
-
-
-
1
-
1
1
1
11
1
1
-
1
-
1
-
-
-
1
-
-
1
-
-