Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.393 extracted from

  • Devlin, A.; Fischbach, M.
    A biosynthetic pathway for a prominent class of microbiota-derived bile acids (2015), Nat. Chem. Biol., 11, 685-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Rumgna_00694, DNA and amino acid sequence determination and analysis, encoded in a three-gene cluster that does not include any additional putative bile acid-metabolic genes, recombinant overvexpression in Escherichia coli [Ruminococcus] gnavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid pH 7.0, 37°C [Ruminococcus] gnavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ [Ruminococcus] gnavus
-
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
-
?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ [Ruminococcus] gnavus ATCC 29149
-
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
-
?
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ [Ruminococcus] gnavus
-
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
-
?
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ [Ruminococcus] gnavus ATCC 29149
-
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
[Ruminococcus] gnavus A7AZH2 gene RUMGNA_00694
-
[Ruminococcus] gnavus ATCC 29149 A7AZH2 gene RUMGNA_00694
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
-
[Ruminococcus] gnavus 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
-
?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
-
[Ruminococcus] gnavus ATCC 29149 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
-
?
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
-
[Ruminococcus] gnavus 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
-
?
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
-
[Ruminococcus] gnavus ATCC 29149 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
3beta-HSDH
-
[Ruminococcus] gnavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at [Ruminococcus] gnavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
157.2
-
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid pH 7.0, 37°C [Ruminococcus] gnavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at [Ruminococcus] gnavus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
[Ruminococcus] gnavus

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids [Ruminococcus] gnavus
physiological function the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides [Ruminococcus] gnavus