Cloned (Comment) | Organism |
---|---|
gene Rumgna_00694, DNA and amino acid sequence determination and analysis, encoded in a three-gene cluster that does not include any additional putative bile acid-metabolic genes, recombinant overvexpression in Escherichia coli | [Ruminococcus] gnavus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid | pH 7.0, 37°C | [Ruminococcus] gnavus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | [Ruminococcus] gnavus | - |
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | - |
? | |
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | [Ruminococcus] gnavus ATCC 29149 | - |
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | - |
? | |
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | [Ruminococcus] gnavus | - |
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | - |
? | |
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | [Ruminococcus] gnavus ATCC 29149 | - |
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Ruminococcus] gnavus | A7AZH2 | gene RUMGNA_00694 | - |
[Ruminococcus] gnavus ATCC 29149 | A7AZH2 | gene RUMGNA_00694 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | - |
[Ruminococcus] gnavus | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | - |
? | |
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | - |
[Ruminococcus] gnavus ATCC 29149 | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | - |
? | |
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | - |
[Ruminococcus] gnavus | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | - |
? | |
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+ | - |
[Ruminococcus] gnavus ATCC 29149 | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSDH | - |
[Ruminococcus] gnavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | [Ruminococcus] gnavus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
157.2 | - |
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid | pH 7.0, 37°C | [Ruminococcus] gnavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | [Ruminococcus] gnavus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
[Ruminococcus] gnavus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids | [Ruminococcus] gnavus |
physiological function | the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides | [Ruminococcus] gnavus |