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Literature summary for 1.1.1.4 extracted from

  • Yu, B.; Sun, J.; Bommareddy, R.R.; Song, L.; Zeng, A.P.
    Novel (2R,3R)-2,3-butanediol dehydrogenase from potential industrial strain Paenibacillus polymyxa ATCC 12321 (2011), Appl. Environ. Microbiol., 77, 4230-4233.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
open reading frame (ORF) scaffold99_orf00067, DNA and amino acid sequence determination and analysis, phylogenetic analysis Paenibacillus polymyxa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
NADH pH 8.0, 22┬░C Paenibacillus polymyxa
0.3
-
(3R,3S)-acetoin pH 8.0, 22┬░C Paenibacillus polymyxa
0.54
-
NAD+ pH 11.0, 22┬░C Paenibacillus polymyxa
1.76
-
(2R,3R)-butane-2,3-diol pH 11.0, 22┬░C Paenibacillus polymyxa
5.62
-
meso-butane-2,3-diol pH 11.0, 22┬░C Paenibacillus polymyxa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+ Paenibacillus polymyxa Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions (3R,3S)-acetoin + NADH + H+
-
r
(2R,3R)-butane-2,3-diol + NAD+ Paenibacillus polymyxa ATCC 12321 Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions (3R,3S)-acetoin + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Paenibacillus polymyxa E7EKB8
-
-
Paenibacillus polymyxa ATCC 12321 E7EKB8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+ Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions Paenibacillus polymyxa (3R,3S)-acetoin + NADH + H+
-
r
(2R,3R)-butane-2,3-diol + NAD+ sole product in the reduction reaction, preferred substrate in the oxidation reaction Paenibacillus polymyxa (3R,3S)-acetoin + NADH + H+ preferred substrate in the reduction reaction r
(2R,3R)-butane-2,3-diol + NAD+ Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions Paenibacillus polymyxa ATCC 12321 (3R,3S)-acetoin + NADH + H+
-
r
(2R,3R)-butane-2,3-diol + NAD+ sole product in the reduction reaction, preferred substrate in the oxidation reaction Paenibacillus polymyxa ATCC 12321 (3R,3S)-acetoin + NADH + H+ preferred substrate in the reduction reaction r
1,2-pentandiol + NAD+
-
Paenibacillus polymyxa ? + NADH + H+
-
r
1,2-propandiol + NAD+
-
Paenibacillus polymyxa ? + NADH + H+
-
r
2-propanol + NAD+
-
Paenibacillus polymyxa 2-propanone + NADH + H+
-
r
2-propanol + NAD+
-
Paenibacillus polymyxa ATCC 12321 2-propanone + NADH + H+
-
r
diacetyl + NADH + H+ 91% activity compared to (3R,3S)-acetoin in the reduction reaction, cf. EC 1.1.1.303 Paenibacillus polymyxa acetoin + NAD+
-
?
diacetyl + NADH + H+ 91% activity compared to (3R,3S)-acetoin in the reduction reaction, cf. EC 1.1.1.303 Paenibacillus polymyxa ATCC 12321 acetoin + NAD+
-
?
glycerol + NAD+
-
Paenibacillus polymyxa ? + NADH + H+
-
r
meso-butane-2,3-diol + NAD+ 72% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction Paenibacillus polymyxa acetoin + NADH + H+
-
r
additional information the enzyme is also active in reduction reaction with diacetyl (91% compared to (2R,3S)-acetoin) and glyceraldehyde-3-phosphate (12% compared to (2R,3S)-acetoin), cf. EC 1.1.1.303 and EC 1.1.1.8, respectively, but not with dihydroxyacetone phosphate. It shows low activity in oxidation reaction with ethanol, n-propanol, n-butanol, 1,3-propanediol, and 1,5-pentanediol Paenibacillus polymyxa ?
-
?
additional information the enzyme is also active in reduction reaction with diacetyl (91% compared to (2R,3S)-acetoin) and glyceraldehyde-3-phosphate (12% compared to (2R,3S)-acetoin), cf. EC 1.1.1.303 and EC 1.1.1.8, respectively, but not with dihydroxyacetone phosphate. It shows low activity in oxidation reaction with ethanol, n-propanol, n-butanol, 1,3-propanediol, and 1,5-pentanediol Paenibacillus polymyxa ATCC 12321 ?
-
?

Synonyms

Synonyms Comment Organism
(2R,3R)-2,3-butanediol dehydrogenase
-
Paenibacillus polymyxa
BDH99::67
-
Paenibacillus polymyxa

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
assay at room temperature Paenibacillus polymyxa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reduction reaction Paenibacillus polymyxa
11
-
oxidation reaction Paenibacillus polymyxa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Paenibacillus polymyxa
NADH
-
Paenibacillus polymyxa

General Information

General Information Comment Organism
evolution the (2R,3R)-2,3-butanediol dehydrogenase belongs to the mostly zinc-containing medium-chain dehydrogenase/reductase superfamily and not to the short-chain dehydrogenase/reductase superfamily, to which meso- and (2S,3S)-2,3-butanediol dehydrogenases belong, phylogenetic analysis. The enzyme contains two hydrophobic residues forming the binding site for cofactor NAD(P), Phe138 and Leu141 (numbers refer to R,R-BDH of Saccharomyces cerevisiae) Paenibacillus polymyxa