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Literature summary for 1.1.1.4 extracted from

  • Zhao, X.; Zhang, X.; Rao, Z.; Bao, T.; Li, X.; Xu, M.; Yang, T.; Yang, S.
    Identification and characterization of a novel 2,3-butanediol dehydrogenase/acetoin reductase from Corynebacterium crenatum SYPA5-5 (2015), Lett. Appl. Microbiol., 61, 573-579.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Corynebacterium crenatum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 1 mM Corynebacterium crenatum
EDTA strong inhibition at 1 mM Corynebacterium crenatum
Fe3+ slight inhibition at 1 mM Corynebacterium crenatum
Na+ slight inhibition at 1 mM Corynebacterium crenatum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ up to 1.5fold increase of activity at 1 mM Corynebacterium crenatum
Mg2+ up to 1.5fold increase of activity at 1 mM Corynebacterium crenatum
Mn2+ up to 1.2fold increase of activity at 1 mM Corynebacterium crenatum
additional information not influenced by Fe2+, NH3+, Ni2+, and Ba2+ Corynebacterium crenatum
Zn2+ up to 1.7fold increase of activity at 1 mM Corynebacterium crenatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE Corynebacterium crenatum

Organism

Organism UniProt Comment Textmining
Corynebacterium crenatum
-
-
-
Corynebacterium crenatum SYPA5-5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography, and gel filtration Corynebacterium crenatum

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Corynebacterium crenatum

Synonyms

Synonyms Comment Organism
2,3-BDH/AR
-
Corynebacterium crenatum
2,3-butanediol dehydrogenase/acetoin reductase
-
Corynebacterium crenatum

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
35
-
-
Corynebacterium crenatum

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
25 40 the enzyme becomes very unstable when the temperature is above 35┬░C with no activity at 40┬░C Corynebacterium crenatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
for reduction reaction Corynebacterium crenatum
10
-
for oxidation reaction Corynebacterium crenatum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Corynebacterium crenatum