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Literature summary for 1.1.1.4 extracted from

  • Yu, M.; Huang, M.; Song, Q.; Shao, J.; Ying, X.
    Characterization of a (2R,3R)-2,3-butanediol dehydrogenase from Rhodococcus erythropolis WZ010 (2015), Molecules, 20, 7156-7173.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
DMSO the addition of 10% (v/v) dimethyl sulfoxide increases the activity by 161.2% Rhodococcus erythropolis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
acetone 38.3% residual activity at 10% (v/v) Rhodococcus erythropolis
acetonitrile 2.4% residual activity at 10% (v/v) Rhodococcus erythropolis
Ag+ complete inhibition at 1 mM Rhodococcus erythropolis
Al3+ 21.6% residual activity at 1 mM Rhodococcus erythropolis
Ba2+ 85.8% residual activity at 1 mM Rhodococcus erythropolis
Ca2+ 89.3% residual activity at 1 mM Rhodococcus erythropolis
Co2+ 79.5% residual activity at 1 mM Rhodococcus erythropolis
Cu2+ complete inhibition at 1 mM Rhodococcus erythropolis
DMSO 8.4% residual activity at 40% (v/v) Rhodococcus erythropolis
EDTA
-
Rhodococcus erythropolis
ethanol 3.7% residual activity at 10% (v/v) Rhodococcus erythropolis
Fe2+ complete inhibition at 1 mM Rhodococcus erythropolis
methanol 14% residual activity at 10% (v/v) Rhodococcus erythropolis
Mn2+ 81.1% residual activity at 1 mM Rhodococcus erythropolis
Zn2+ 3.4% residual activity at 1 mM Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
NADH with diacetyl as cosubstrate, at pH 10.0 and 45┬░C Rhodococcus erythropolis
0.1
-
diacetyl at pH 10.0 and 45┬░C Rhodococcus erythropolis
0.58
-
(2R,3R)-butane-2,3-diol at pH 6.5 and 55┬░C Rhodococcus erythropolis
1.04
-
NAD+ with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55┬░C Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 138.9% activity at 1 mM Rhodococcus erythropolis
additional information Na+ and Mg2+ cause no significant activation Rhodococcus erythropolis
Zn2+ a zinc-containing alcohol dehydrogenase Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
-
Rhodococcus erythropolis
45000
-
gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+ Rhodococcus erythropolis
-
(R)-acetoin + NADH + H+
-
r
(R)-acetoin + NADH + H+ Rhodococcus erythropolis the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 (2R,3R)-butane-2,3-diol + NAD+
-
r
(R)-acetoin + NADH + H+ Rhodococcus erythropolis WZ010 the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 (2R,3R)-butane-2,3-diol + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis A0A0E4A9D6
-
-
Rhodococcus erythropolis WZ010 A0A0E4A9D6
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+
-
Rhodococcus erythropolis (R)-acetoin + NADH + H+
-
r
(2R,3R)-butane-2,3-diol + NAD+ 69.5% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
(R)-1-phenyl-1,2-ethanediol + NAD+ 32.2% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
(R)-acetoin + NADH + H+ the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 Rhodococcus erythropolis (2R,3R)-butane-2,3-diol + NAD+
-
r
(R)-acetoin + NADH + H+ the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 Rhodococcus erythropolis WZ010 (2R,3R)-butane-2,3-diol + NAD+
-
r
2,2,2-trifluoroacetophenone + NADH + H+ 28% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
2,2,2-trifluoroacetophenone + NADH + H+ 28% activity compared to (R)-acetoin Rhodococcus erythropolis WZ010 ?
-
?
2-hydroxyacetophenone + NADH + H+ 8.7% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
2-octanone + NADH + H+ 9.3% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
3-methyl-2-butenal + NADH + H+ 16.7% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
butane-1,3-diol + NAD+ 14.7% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
diacetyl + NADH + H+ 69.3% activity compared to (R)-acetoin Rhodococcus erythropolis (R)-acetoin + NAD+
-
r
diacetyl + NADH + H+ 69.3% activity compared to (R)-acetoin Rhodococcus erythropolis WZ010 (R)-acetoin + NAD+
-
r
ethyl 4-chloro-3-hydroxybutyrate + NAD+ 12.2% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
glycerol + NAD+ 16.3% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
glycerol + NAD+ 16.3% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis WZ010 ?
-
?
additional information no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone Rhodococcus erythropolis ?
-
?
additional information no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone Rhodococcus erythropolis WZ010 ?
-
?
sodium lactate + NAD+ 10.2% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37180, calculated from amino acid sequence Rhodococcus erythropolis
monomer 1 * 44000, His-tagged enzyme, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
(2R,3R)-2,3-butanediol dehydrogenase
-
Rhodococcus erythropolis
BDH
-
Rhodococcus erythropolis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
for (2R,3R)-butane-2,3-diol oxidation Rhodococcus erythropolis
55
-
for acetoin reduction Rhodococcus erythropolis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
after 6 h incubation at 45┬░C, the remaining activities for acetoin reduction and butane-2,3-diol oxidation are 49.7% and 43.5%, respectively Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.28
-
(2R,3R)-butane-2,3-diol at pH 6.5 and 55┬░C Rhodococcus erythropolis
5.73
-
NADH with diacetyl as cosubstrate, at pH 10.0 and 45┬░C Rhodococcus erythropolis
6.11
-
diacetyl at pH 10.0 and 45┬░C Rhodococcus erythropolis
10.27
-
NAD+ with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55┬░C Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
for acetoin reduction Rhodococcus erythropolis
10
-
for (2R,3R)-butane-2,3-diol oxidation Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7 for the reduction of acetoin, more than 85% of its maximal activity is in the pH range of 6.5 to 7.0 Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhodococcus erythropolis
NADH
-
Rhodococcus erythropolis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.379
-
(2R,3R)-butane-2,3-diol at pH 6.5 and 55┬░C Rhodococcus erythropolis
9.875
-
NAD+ with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55┬░C Rhodococcus erythropolis
61.1
-
diacetyl at pH 10.0 and 45┬░C Rhodococcus erythropolis
71.63
-
NADH with diacetyl as cosubstrate, at pH 10.0 and 45┬░C Rhodococcus erythropolis