Literature summary for 1.1.1.4 extracted from

Zhang, X.; Han, R.; Bao, T.; Zhao, X.; Li, X.; Zhu, M.; Yang, T.; Xu, M.; Shao, M.; Zhao, Y.; Rao, Z.
Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method (2019), Microb. Cell Fact., 18, 128 .

Search for more literature for 1.1.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
gene bdhA, recombinant eenzyme expression in non-natural AC and 2,3-BD producing Corynebacterium crenatum derived from wild-type strain SYPA5-5, coexpression of 2,3-BD biosynthesis pathway genes alsS, alsD, and bdhA	Bacillus subtilis subsp. subtilis

Protein Variants

Protein Variants	Comment	Organism
additional information	coexpression of 2,3-BD biosynthesis pathway genes alsS, alsD, and bdhA in synthetically engineered strains Corynebacterium crenatumDELTAbutADELTAldh SD and Corynebacterium crenatumDELTAldh SDA for selective acetoin (AC) and 2,3-butanediol (2,3-BD) production. Production of AC and 2,3-BD using resting cell bioconversion with glucose as substrate, method evaluation, overview	Bacillus subtilis subsp. subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R,R)-butane-2,3-diol + NAD+	Bacillus subtilis subsp. subtilis	-	(R)-acetoin + NADH + H+	-	r
(R,R)-butane-2,3-diol + NAD+	Bacillus subtilis subsp. subtilis 168	-	(R)-acetoin + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis subsp. subtilis	O34788	-	-
Bacillus subtilis subsp. subtilis 168	O34788	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis subsp. subtilis	(R)-acetoin + NADH + H+	-	r
(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis subsp. subtilis 168	(R)-acetoin + NADH + H+	-	r

Synonyms

Synonyms	Comment	Organism
2,3-butanediol dehydrogenase	-	Bacillus subtilis subsp. subtilis
BdhA	-	Bacillus subtilis subsp. subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	in vivo assay at	Bacillus subtilis subsp. subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Bacillus subtilis subsp. subtilis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bacillus subtilis subsp. subtilis
NADH	-	Bacillus subtilis subsp. subtilis

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Release 2023.1 (January 2023)