BRENDA - Enzyme Database
show all sequences of 1.1.1.4

Effect of deletion of 2,3-butanediol dehydrogenase gene (bdhA) on acetoin production of Bacillus subtilis

Zhang, J.; Zhao, X.; Zhang, J.; Zhao, C.; Liu, J.; Tian, Y.; Yang, L.; Prep. Biochem. Biotechnol. 47, 761-767 (2017) View publication on PubMed

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
oxygen
oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield
Bacillus subtilis
Cloned(Commentary)
Cloned (Commentary)
Organism
gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol
Bacillus subtilis
Engineering
Protein Variants
Commentary
Organism
additional information
construction of an engineered Bacillus subtilis strain 168 in which the bdhA gene is knocked out by the cre/lox system using the lox71-zeo-lox66 resistance marker cassette. The effects of bdhA gene deletion on production of acetoin and 2,3-butanediol are evaluated. By increasing the glucose concentration, the acetoin yield is improved from 6.61 g/l to 24.6 g/l. Deletion of the gene bdhA efficiently blocks the transformation of acetoin and 2,3-butanediol during the fermentation of strain BS168D, overview
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R,R)-butane-2,3-diol + NAD+
Bacillus subtilis
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Bacillus subtilis BS168D
-
(R)-acetoin + NADH + H+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
O34788
-
-
Bacillus subtilis BS168D
O34788
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
cultivation of wild-type and mutant strains at pH 7.0, 37°C
Bacillus subtilis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R,R)-butane-2,3-diol + NAD+
-
762259
Bacillus subtilis
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
762259
Bacillus subtilis BS168D
(R)-acetoin + NADH + H+
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
2,3-butanediol dehydrogenase
-
Bacillus subtilis
BDH
-
Bacillus subtilis
BdhA
-
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus subtilis
NADH
-
Bacillus subtilis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
oxygen
oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus subtilis
NADH
-
Bacillus subtilis
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of an engineered Bacillus subtilis strain 168 in which the bdhA gene is knocked out by the cre/lox system using the lox71-zeo-lox66 resistance marker cassette. The effects of bdhA gene deletion on production of acetoin and 2,3-butanediol are evaluated. By increasing the glucose concentration, the acetoin yield is improved from 6.61 g/l to 24.6 g/l. Deletion of the gene bdhA efficiently blocks the transformation of acetoin and 2,3-butanediol during the fermentation of strain BS168D, overview
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R,R)-butane-2,3-diol + NAD+
Bacillus subtilis
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Bacillus subtilis BS168D
-
(R)-acetoin + NADH + H+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
cultivation of wild-type and mutant strains at pH 7.0, 37°C
Bacillus subtilis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R,R)-butane-2,3-diol + NAD+
-
762259
Bacillus subtilis
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
762259
Bacillus subtilis BS168D
(R)-acetoin + NADH + H+
-
-
-
r
General Information
General Information
Commentary
Organism
malfunction
deletion of bdhA gene successfully blocks the reversible transformation between acetoin and 2,3-butanediol and eliminates the effect of dissolved oxygen on the transformation
Bacillus subtilis
physiological function
acetoin and 2,3-butanediol can be transformed into each other by 2,3-butanediol dehydrogenase (BDH) using NADH/NAD+ as coenzyme. The main 2,3-butanediol production of strain BS168D is meso-2,3-butanediol and the bdhA gene is only responsible for (2R,3R)-2,3-butanediol synthesis. Oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion of bdhA gene successfully blocks the reversible transformation between acetoin and 2,3-butanediol and eliminates the effect of dissolved oxygen on the transformation
Bacillus subtilis
physiological function
acetoin and 2,3-butanediol can be transformed into each other by 2,3-butanediol dehydrogenase (BDH) using NADH/NAD+ as coenzyme. The main 2,3-butanediol production of strain BS168D is meso-2,3-butanediol and the bdhA gene is only responsible for (2R,3R)-2,3-butanediol synthesis. Oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield
Bacillus subtilis
Other publictions for EC 1.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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-
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1
-
2
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-
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1
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-
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8
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1
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1
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2
1
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1
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1
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1
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16
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11
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1
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2
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1
2
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1
-
1
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16
-
-
-
-
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8
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48
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1
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-
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1
-
-
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-
2
2
-
-
-
760411
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Paenibacillus brasilensis, Paenibacillus brasilensis PB24
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2
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2
-
-
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-
-
-
-
4
-
4
-
-
-
-
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-
-
1
-
-
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1
1
1
-
-
-
-
-
-
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-
2
-
-
-
-
-
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-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
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Liang
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39
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-
-
-
-
-
-
-
2
-
-
-
-
-
32
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
2
-
-
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2
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-
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2
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-
-
-
-
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4
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-
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2
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1
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-
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1
-
1
-
-
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2
-
3
-
-
-
-
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1
-
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2
-
4
-
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2
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1
2
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1
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2
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1
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2
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1
3
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-
6
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2
1
-
1
-
1
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1
2
-
-
-
-
1
1
2
-
1
-
-
-
-
2
-
-
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2
1
-
-
1
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-
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-
6
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1
-
1
-
1
-
1
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
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2
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5
-
-
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2
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3
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2
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1
2
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1
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2
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-
-
2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
762259
Zhang
Effect of deletion of 2,3-but ...
Bacillus subtilis, Bacillus subtilis BS168D
Prep. Biochem. Biotechnol.
47
761-767
2017
1
-
1
-
1
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2
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5
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1
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2
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3
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2
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1
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2
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1
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2
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1
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2
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2
2
-
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-
737512
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100
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2016
-
-
1
-
-
-
-
4
-
-
-
4
-
37
-
-
1
-
-
-
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10
-
3
-
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-
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1
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-
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1
1
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4
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4
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1
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10
-
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-
-
-
-
-
-
-
-
-
-
4
4
737519
de Oliveira
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100
719-728
2016
-
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1
-
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-
-
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2
2
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8
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2
2
1
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1
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1
1
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2
2
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2
2
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-
-
-
-
-
-
738345
Ge
-
Contracted but effective: Prod ...
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18
4693-4703
2016
-
-
1
-
-
-
-
-
-
-
-
4
-
3
-
-
1
-
-
-
14
-
20
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
14
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739694
Bae
Efficient production of acetoi ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
Sci. Rep.
6
27667
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
76
-
-
-
-
-
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-
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1
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1
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1
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-
737847
Kang
Enhanced production of 2,3-but ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) BRC1
Bioprocess Biosyst. Eng.
38
299-305
2015
-
-
1
-
-
-
-
4
-
1
-
2
-
2
-
-
1
-
-
-
-
-
14
-
2
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
1
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
-
2
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246407
Höhn-Bentz
Bacterial 2,3-butanediol dehyd ...
Aeromonas hydrophila, Bacillus subtilis, Gluconobacter oxydans, Paenibacillus polymyxa
Arch. Microbiol.
116
197-203
1978
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