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Literature summary for 1.1.1.4 extracted from

  • Zhang, J.; Zhao, X.; Zhang, J.; Zhao, C.; Liu, J.; Tian, Y.; Yang, L.
    Effect of deletion of 2,3-butanediol dehydrogenase gene (bdhA) on acetoin production of Bacillus subtilis (2017), Prep. Biochem. Biotechnol., 47, 761-767 .
    View publication on PubMed
Search for more literature for 1.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
oxygen oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information construction of an engineered Bacillus subtilis strain 168 in which the bdhA gene is knocked out by the cre/lox system using the lox71-zeo-lox66 resistance marker cassette. The effects of bdhA gene deletion on production of acetoin and 2,3-butanediol are evaluated. By increasing the glucose concentration, the acetoin yield is improved from 6.61 g/l to 24.6 g/l. Deletion of the gene bdhA efficiently blocks the transformation of acetoin and 2,3-butanediol during the fermentation of strain BS168D, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R,R)-butane-2,3-diol + NAD+ Bacillus subtilis
-
 (R)-acetoin + NADH + H+
-
 r
(R,R)-butane-2,3-diol + NAD+ Bacillus subtilis BS168D
-
 (R)-acetoin + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34788
-
-
Bacillus subtilis BS168D O34788
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information cultivation of wild-type and mutant strains at pH 7.0, 37°C Bacillus subtilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,R)-butane-2,3-diol + NAD+
-
 Bacillus subtilis (R)-acetoin + NADH + H+
-
 r
(R,R)-butane-2,3-diol + NAD+
-
 Bacillus subtilis BS168D (R)-acetoin + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase
-
 Bacillus subtilis
BDH
-
 Bacillus subtilis
BdhA
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NADH
-
 Bacillus subtilis

General Information

General Information Comment Organism
malfunction deletion of bdhA gene successfully blocks the reversible transformation between acetoin and 2,3-butanediol and eliminates the effect of dissolved oxygen on the transformation Bacillus subtilis
physiological function acetoin and 2,3-butanediol can be transformed into each other by 2,3-butanediol dehydrogenase (BDH) using NADH/NAD+ as coenzyme. The main 2,3-butanediol production of strain BS168D is meso-2,3-butanediol and the bdhA gene is only responsible for (2R,3R)-2,3-butanediol synthesis. Oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield Bacillus subtilis