Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | inhibition is much more pronounced in the mitochondrial enzyme than in the cytosolic enzyme and occurs at physiological acetyl-CoA concentrations | Sus scrofa | |
malonyl-CoA | inhibition of mitochondrial enzyme and cytosolic enzyme to a much lower extent than with acetyl-CoA | Sus scrofa | |
Mg2+ | mitochondrial enzyme, decarboxylation reaction, above 6 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00118 | - |
NADP+ | cytosolic enzyme | Sus scrofa | |
0.00188 | - |
NADP+ | mitochondrial enzyme | Sus scrofa | |
0.14 | - |
L-malate | cytosolic enzyme | Sus scrofa | |
1.25 | - |
L-malate | mitochondrial enzyme | Sus scrofa | |
10 | - |
pyruvate | cytosolic enzyme | Sus scrofa | |
25 | - |
pyruvate | mitochondrial enzyme | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | 2 distinct enzyme forms are present in cytosol and in mitochondria | Sus scrofa | 5829 | - |
mitochondrion | 2 distinct enzyme forms are present in cytosol and in mitochondria | Sus scrofa | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity of cytosolic enzyme and mitochondrial enzyme is strictly dependent on | Sus scrofa | |
Mg2+ | optimal concentration: 6 mM for the decarboxylation of the mitochondrial enzyme, 20 mM for the cytosolic enzyme | Sus scrofa | |
Mn2+ | activity of cytosolic enzyme and mitochondrial enzyme is strictly dependent on | Sus scrofa | |
Mn2+ | optimal concentration is 15 mM for the cytosolic enzyme and 10 mM for the mitochondrial enzyme | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
216000 | - |
cytosolic enzyme, density gradient centrifugation | Sus scrofa |
227000 | - |
mitochondrial enzyme, density gradient centrifugation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 distinct enzyme forms | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac muscle | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Sus scrofa | pyruvate + CO2 + NADPH | - |
r | |
pyruvate + CO2 + NADPH | - |
Sus scrofa | L-malate + NADP+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
mitochondrial enzyme, carboxylation | Sus scrofa |
7 | - |
carboxylation and decarboxylation, cytosolic enzyme | Sus scrofa |
7.6 | - |
cytosolic enzyme, decarboxylation | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor | Sus scrofa | |
NADPH | cofactor | Sus scrofa |