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Literature summary for 1.1.1.40 extracted from

  • Zelewski, M.; Swierczynski, J.
    Malic enzyme in human liver. Intracellular distribution, purification and properties of cytosolic isozyme (1991), Eur. J. Biochem., 201, 339-345.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0053
-
NADPH
-
Homo sapiens
0.0092
-
NADP+
-
Homo sapiens
0.12
-
L-malate with NADP+ as cosubstrate Homo sapiens
0.96
-
L-malate with NAD+ as cosubstrate Homo sapiens
4.6
-
NAD+
-
Homo sapiens
5.9
-
pyruvate
-
Homo sapiens
27.9
-
CO2
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
4 * 63000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56.35
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ at 39% of the activity with NADP+ Homo sapiens pyruvate + CO2 + NADH
-
ir
(S)-malate + NADP+ the reverse reaction is catalyzed at 84% of the activity Homo sapiens pyruvate + CO2 + NADPH
-
r
Oxaloacetate at 1.3% of the rate of NADP+-linked oxidative decarboxylation Homo sapiens Pyruvate + CO2
-
?
pyruvate + CO2 + NADPH at 84% of the activity of the NADP+-linked oxidative decarboxylation of malate Homo sapiens L-malate + NADP+
-
r
pyruvate + CO2 + NADPH
-
Homo sapiens (S)-malate + NADP+
-
r
pyruvate + NADPH at 5.4% of the activity of NADP-dependent oxidative decarboxylation of malate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 63000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
NADP+-linked decarboxylation of malate, at 0.2 mM malate Homo sapiens
7.3
-
carboxylation of pyruvate Homo sapiens
7.3
-
NADP+-linked decarboxylation of malate, at 1 mM malate Homo sapiens
8
-
NADP+-linked decarboxylation of malate, at 10 mM malate Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Homo sapiens
NADPH cofactor Homo sapiens