Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+-ascorbate | rapid inactivation by generation of reactive oxygen species at pH 5.0, Fe2+ can substitute for Cu2+, Cu2+ or ascorbate alone are not effective, azide, 1,4-diazabicyclo-(2.2.2.)octane, histidine and imidazole protect against inhibition, the substrates L-malate and NADP+ and EDTA protect almost completely, loss of activity is accompanied with cleavage of the protein into 4 fragments of 14-55 kDa | Zea mays | |
Fe2+-ascorbate | rapid inactivation | Zea mays | |
H2O2 | 83% inhibition at 0.25 mM, 91.1% inhibition at 0.5 mM | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
4 * 62000, SDS-PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62000, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Zea mays |