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Literature summary for 1.1.1.40 extracted from

  • Shin, J.A.; Kwon, Y.D.; Kwon, O.H.; Lee, H.S.; Kim, P.
    5-aminolevulinic acid biosynthesis in Escherichia coli coexpressing NADP-dependent malic enzyme and 5-aminolevulinate synthase (2007), J. Microbiol. Biotechnol., 17, 1579-1584.
    View publication on PubMed
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
co-expression in strain W3110 with 5-aminolevulinate synthase, EC 2.3.1.37, from Rhodobacter sphaeroides, hemA gene, for reconstruction of the 5-aminolevulinic acid biosynthesis, subcloning in strain DH5alpha, increased C4 metabolism via NADP-dependent malic enzyme expression results in increased 5-aminolevulinate production by 5-aminolevulinate synthase in Escherichia coli Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
NH4Cl activates Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Escherichia coli
-
 pyruvate + CO2 + NADPH
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene maeB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Escherichia coli pyruvate + CO2 + NADPH
-
 r

Synonyms

Synonyms Comment Organism
MaeB
-
 Escherichia coli
NADP-dependent malic enzyme
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli
NADPH
-
 Escherichia coli