Cloned (Comment) | Organism |
---|---|
gene maeB, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of gene maeB by chromosomal homologous recombination | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Escherichia coli | |
Co2+ | - |
Escherichia coli | |
Cu2+ | strong inhibition | Escherichia coli | |
Zn2+ | strong inhibition | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, can partially substitute for Mn2+ to about 92% of the maximal activity | Escherichia coli | |
Mn2+ | dependent on, best at 20 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
x * 83000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Escherichia coli | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Escherichia coli MG1655 | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P76558 | gene maeB | - |
Escherichia coli MG1655 | P76558 | gene maeB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.15 | - |
pH 7.8, 46°C, purified recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Escherichia coli MG1655 | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
MaeB | - |
Escherichia coli |
NADP-dependent malic enzyme | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | activity range, profile overview | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | activity range, profile overview | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Escherichia coli |