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Literature summary for 1.1.1.401 extracted from

  • Reinhardt, A.; Johnsen, U.; Schoenheit, P.
    L-Rhamnose catabolism in archaea (2019), Mol. Microbiol., 111, 1093-1108 .
    View publication on PubMed
Search for more literature for 1.1.1.401

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli rosetta Vulcanisaeta distributa
homologous overexpression in Haloferax volcanii H1209 Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 NAD+ 42°C. pH 8.0 Haloferax volcanii
0.66
-
 2-dehydro-3-deoxy-L-rhamnonate 42°C. pH 8.0 Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
139000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-L-rhamnonate + NAD+ Haloferax volcanii
-
 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
2-dehydro-3-deoxy-L-rhamnonate + NAD+ Vulcanisaeta distributa
-
 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
2-dehydro-3-deoxy-L-rhamnonate + NAD+ Haloferax volcanii ATCC 29605
-
 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GPB2
-
-
Haloferax volcanii ATCC 29605 D4GPB2
-
-
Vulcanisaeta distributa E1QSX6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax volcanii
-
 Vulcanisaeta distributa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
 Haloferax volcanii 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
 Vulcanisaeta distributa 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
 Haloferax volcanii ATCC 29605 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 46600, SDS-PAGE Haloferax volcanii

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-L-rhamnonate dehydrogenase
-
 Haloferax volcanii
2-keto-3-deoxy-L-rhamnonate dehydrogenase
-
 Vulcanisaeta distributa
KDRDH
-
 Haloferax volcanii
KDRDH
-
 Vulcanisaeta distributa
Vdis_1457
-
 Vulcanisaeta distributa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Vulcanisaeta distributa
NAD+ the enzyme does not show activity with NADP+ Haloferax volcanii

General Information

General Information Comment Organism
malfunction deletion mutant does not grow L-rhamnose Haloferax volcanii