Data extracted from this reference:
Cloned(Commentary)
spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148
Streptococcus pneumoniae
KM Value [mM]
0.061
D-ribulose 5-phosphate
Streptococcus pneumoniae
Organism
Streptococcus pneumoniae
Q8DPI3
Purification (Commentary)
by gel filtration
Streptococcus pneumoniae
Storage Stability
-20°C
Streptococcus pneumoniae
Substrates and Products (Substrate)
D-ribulose 5-phosphate + NADPH + H+
698603
Streptococcus pneumoniae
D-ribitol 5-phosphate + NADP+
?
Turnover Number [1/s]
0.5
D-ribulose 5-phosphate
Streptococcus pneumoniae
Cofactor
additional information
NADH is not consumed in the presence of Spr1148-His and ribulose 5-phosphate
Streptococcus pneumoniae
NADPH
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148
Streptococcus pneumoniae
Cofactor (protein specific)
additional information
NADH is not consumed in the presence of Spr1148-His and ribulose 5-phosphate
Streptococcus pneumoniae
NADPH
Streptococcus pneumoniae
KM Value [mM] (protein specific)
0.061
D-ribulose 5-phosphate
Streptococcus pneumoniae
Purification (Commentary) (protein specific)
by gel filtration
Streptococcus pneumoniae
Storage Stability (protein specific)
-20°C
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
D-ribulose 5-phosphate + NADPH + H+
698603
Streptococcus pneumoniae
D-ribitol 5-phosphate + NADP+
?
Turnover Number [1/s] (protein specific)
0.5
D-ribulose 5-phosphate
Streptococcus pneumoniae
Other publictions for EC 1.1.1.405
698603
Baur
Synthesis of CDP-activated rib ...
Streptococcus pneumoniae
J. Bacteriol.
191
1200-1210
2009
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
-
-
-
1
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
650236
Pereira
Bifunctional catalysis by CDP- ...
Haemophilus influenzae, Staphylococcus aureus
Biochemistry
43
11802-11812
2004
-
-
1
-
-
-
6
4
-
-
5
-
-
2
-
-
1
-
-
-
-
-
2
2
-
-
-
4
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
6
2
4
-
-
5
-
-
-
-
1
-
-
-
-
2
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
645254
Zolli
Reduction precedes cytidylyl t ...
Haemophilus influenzae
Biochemistry
40
5041-5048
2001
-
-
1
-
2
-
-
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
9
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
9
-
-
-
-
-
-
-
-
11
11
645253
Follens
acs1 of Haemophilus influenzae ...
Haemophilus influenzae
J. Bacteriol.
181
2001-2007
1999
2
-
1
-
-
1
1
3
-
-
1
-
-
1
-
-
-
-
-
-
2
-
3
1
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
-
-
2
-
3
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-