show 
Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae
Baur, S.; Marles-Wright, J.; Buckenmaier, S.; Lewis, R.J.; Vollmer, W.; J. Bacteriol. 191, 1200-1210 (2009)
Data extracted from this reference:
Cloned(Commentary)
Commentary
Organism
spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148
Streptococcus pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
D-ribulose 5-phosphate
-
Streptococcus pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae
Q8DPI3
-
-
Purification (Commentary)
Commentary
Organism
by gel filtration
Streptococcus pneumoniae
Storage Stability
Storage Stability
Organism
-20°C
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-ribulose 5-phosphate + NADPH + H+
-
698603
Streptococcus pneumoniae
D-ribitol 5-phosphate + NADP+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.5
-
D-ribulose 5-phosphate
-
Streptococcus pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
NADH is not consumed in the presence of Spr1148-His and ribulose 5-phosphate
Streptococcus pneumoniae
NADPH
-
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148
Streptococcus pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
NADH is not consumed in the presence of Spr1148-His and ribulose 5-phosphate
Streptococcus pneumoniae
NADPH
-
Streptococcus pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
D-ribulose 5-phosphate
-
Streptococcus pneumoniae
Purification (Commentary) (protein specific)
Commentary
Organism
by gel filtration
Streptococcus pneumoniae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-ribulose 5-phosphate + NADPH + H+
-
698603
Streptococcus pneumoniae
D-ribitol 5-phosphate + NADP+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.5
-
D-ribulose 5-phosphate
-
Streptococcus pneumoniae
Other publictions for EC 1.1.1.405
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698603
Baur
Synthesis of CDP-activated rib ...
Streptococcus pneumoniae
J. Bacteriol.
191
1200-1210
2009
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650236
Pereira
Bifunctional catalysis by CDP- ...
Haemophilus influenzae, Staphylococcus aureus
Biochemistry
43
11802-11812
2004
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645254
Zolli
Reduction precedes cytidylyl t ...
Haemophilus influenzae
Biochemistry
40
5041-5048
2001
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12
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645253
Follens
acs1 of Haemophilus influenzae ...
Haemophilus influenzae
J. Bacteriol.
181
2001-2007
1999
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