Application | Comment | Organism |
---|---|---|
synthesis | synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol using an immobilized enzyme system. One-pot purification of His6-tagged GatDH and FDH followed by the production of rare sugar and chiral diol by use of affinity magnetic nanoparticles | Cereibacter sphaeroides |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21GOLD(DE3) | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | rapid immobilization with the one-pot purification of galactitol dehydrogenase and formate dehydrogenase, using iminodiacetic acid with chelated Co2+ modified magnetic nanoparticles as a carrier, for (S)-1,2-propanediol and L-tagatose production. Lactate dehydrogenase from recombinant Escherichia coli and formate dehydrogenase from Candida methylica are used for the regeneration of NADH/NAD+ with a representative synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol, overview. The immobilized enzyme system retained up to 70% of its activity after one week of repeated use. One-pot purification of His6-tagged GatDH and FDH followed by the production of rare sugar and chiral diol by use of affinity magnetic nanoparticles | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | C0KTJ6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His(6)-tagged GatDH from Escherichia coli strain BL21GOLD(DE3) by use of Co2+-IDA functionalized Fe2O3 nanoparticles, purification and immobilization method, overview | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactitol + NAD+ | - |
Cereibacter sphaeroides | L-tagatose + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GatDH | - |
Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cereibacter sphaeroides | |
NADH | - |
Cereibacter sphaeroides |