Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens | |
citrate | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon-Plus cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
alphabeta heterodimer with the alpha subunit in apo form and in Ca2_-bound, NAD-bound, and NADH-bound forms, hanging drop vapor diffusion method, using either containing 8% (v/v) tacsimate (pH 6.0) and 20% (w/v) PEG 3350 or 0.2 M calcium acetate (pH 7.5) and 20% (w/v) PEG 3350 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH can compete with NAD+ to bind to the active site and inhibits the activity of the alphabeta heterodimer | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Homo sapiens | |
Mn2+ | 2 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50213 and O43837 | subunits alpha and beta | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent isocitrate dehydrogenase | - |
Homo sapiens |
NAD-IDH | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |