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Literature summary for 1.1.1.41 extracted from

  • Wang, X.; Inaoka, D.K.; Shiba, T.; Balogun, E.O.; Allmann, S.; Watanabe, Y.I.; Boshart, M.; Kita, K.; Harada, S.
    Expression, purification, and crystallization of type 1 isocitrate dehydrogenase from Trypanosoma brucei (2017), Protein Expr. Purif., 138, 56-62 .
    View publication on PubMed
Search for more literature for 1.1.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21star(DE3) cells Trypanosoma brucei brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer, pH 7.2, 200 mM NaCl and 22% (w/v) PEG6000 Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Trypanosoma brucei brucei 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mM used in assay conditions Trypanosoma brucei brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97500
-
 gel filtration Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Trypanosoma brucei brucei
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NAD+ Trypanosoma brucei brucei ILtat 1.4
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
-
-
-
Trypanosoma brucei brucei ILtat 1.4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Trypanosoma brucei brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.7
-
 with NADP+ as cosubstrate, at pH 8.0, temperature not specified in the publication Trypanosoma brucei brucei
22.1
-
 with NAD+ as cosubstrate, at pH 8.0, temperature not specified in the publication Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
 Trypanosoma brucei brucei 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NAD+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NAD+
-
 Trypanosoma brucei brucei ILtat 1.4 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NAD+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei ILtat 1.4 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NADP+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
isocitrate + NADP+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei ILtat 1.4 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, His6-SUMO-tagged enzyme, SDS-PAGE Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
IDH1
-
 Trypanosoma brucei brucei
NAD+-dependent type 1 IDH
-
 Trypanosoma brucei brucei
type 1 isocitrate dehydrogenase
-
 Trypanosoma brucei brucei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Trypanosoma brucei brucei