Literature summary for 1.1.1.41 extracted from

Liu, Y.; Hu, L.; Ma, T.; Yang, J.; Ding, J.
Insights into the inhibitory mechanisms of NADH on the alphagamma heterodimer of human NAD-dependent isocitrate dehydrogenase (2018), Sci. Rep., 8, 3146 .

Search for more literature for 1.1.1.41

Activating Compound

Activating Compound	Comment	Organism	Structure
ADP	-	Homo sapiens
citrate	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Codon-Plus cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
alphagamma heterodimer bound with an Mg2+ and an NADH at the active site and an NADH at the allosteric site, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 50 mM MgCl2, and 30% (w/v) PEGMME 550	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
NADH	NADH at the active site occupies the binding site for NAD+ and prevents the binding of the cofactor. NADH at the allosteric site occupies the binding sites for ADP and citrate and blocks the binding of the activators. NADH inhibits the activities of the alphagamma, alphabeta and alpha2betagamma enzyme forms	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	2 mM used in assay conditions	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isocitrate + NAD+	Homo sapiens	-	2-oxoglutarate + CO2 + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P50213 and O43837 and P51553	subunits alpha, beta and gamma	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isocitrate + NAD+	-	Homo sapiens	2-oxoglutarate + CO2 + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
NAD-dependent isocitrate dehydrogenase	-	Homo sapiens
NAD-IDH	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0785	-	alpha2betagamma heterotetramer, at pH 7.4 and 25°C	Homo sapiens	NADH
0.0827	-	alphagamma heterodimer, at pH 7.4 and 25°C	Homo sapiens	NADH
0.0949	-	alphabeta heterodimer, at pH 7.4 and 25°C	Homo sapiens	NADH

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Release 2023.1 (January 2023)