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Literature summary for 1.1.1.414 extracted from

  • Hobbs, M.E.; Williams, H.J.; Hillerich, B.; Almo, S.C.; Raushel, F.M.
    L-Galactose metabolism in Bacteroides vulgatus from the human gut microbiota (2014), Biochemistry, 53, 4661-4670 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.414

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Phocaeicola vulgatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 L-galactonate pH 8.0, 30°C Phocaeicola vulgatus
0.51
-
 L-gluconate pH 8.0, 30°C Phocaeicola vulgatus
0.55
-
 D-tagaturonate pH 7.0, 30°C Phocaeicola vulgatus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+-dependent enzyme Phocaeicola vulgatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactonate + NAD+ Phocaeicola vulgatus the enzyme participates in an L-galactonate degradation pathway D-tagaturonate + NADH + H+
-
 ?
L-galactonate + NAD+ Phocaeicola vulgatus DSM 1447 the enzyme participates in an L-galactonate degradation pathway D-tagaturonate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Phocaeicola vulgatus A6KWY5
-
-
Phocaeicola vulgatus DSM 1447 A6KWY5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phocaeicola vulgatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tagaturonate + NADH + H+
-
 Phocaeicola vulgatus L-galactonate + NAD+
-
 ?
D-tagaturonate + NADH + H+
-
 Phocaeicola vulgatus DSM 1447 L-galactonate + NAD+
-
 ?
L-fuconate + NAD+
-
 Phocaeicola vulgatus ?
-
 ?
L-fuconate + NAD+
-
 Phocaeicola vulgatus DSM 1447 ?
-
 ?
L-galactonate + NAD+
-
 Phocaeicola vulgatus D-tagaturonate + NADH + H+
-
 ?
L-galactonate + NAD+ the enzyme participates in an L-galactonate degradation pathway Phocaeicola vulgatus D-tagaturonate + NADH + H+
-
 ?
L-galactonate + NAD+
-
 Phocaeicola vulgatus DSM 1447 D-tagaturonate + NADH + H+
-
 ?
L-galactonate + NAD+ the enzyme participates in an L-galactonate degradation pathway Phocaeicola vulgatus DSM 1447 D-tagaturonate + NADH + H+
-
 ?
L-gluconate + NAD+ the value of kcat/Km for the oxidation of L-galactonate is 2 orders of magnitude greater than the oxidation of L-gluconate Phocaeicola vulgatus ?
-
 ?
additional information no oxidation of D-arabinonate and L-xylonate Phocaeicola vulgatus ?
-
 ?
additional information no oxidation of D-arabinonate and L-xylonate Phocaeicola vulgatus DSM 1447 ?
-
 ?

Synonyms

Synonyms Comment Organism
Bvu0222
-
 Phocaeicola vulgatus
L-galactonate dehydrogenase
-
 Phocaeicola vulgatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Phocaeicola vulgatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 L-gluconate pH 8.0, 30°C Phocaeicola vulgatus
0.6
-
 L-galactonate pH 8.0, 30°C Phocaeicola vulgatus
90
-
 D-tagaturonate pH 7.0, 30°C Phocaeicola vulgatus

General Information

General Information Comment Organism
metabolism the enzyme participates in an L-galactonate degradation pathway Phocaeicola vulgatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.17
-
 L-galactonate pH 8.0, 30°C Phocaeicola vulgatus
0.2
-
 L-fuconate pH 8.0, 30°C Phocaeicola vulgatus
0.39
-
 L-gluconate pH 8.0, 30°C Phocaeicola vulgatus
160
-
 D-tagaturonate pH 7.0, 30°C Phocaeicola vulgatus