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Literature summary for 1.1.1.42 extracted from
- Purification, properties and enhanced expression under nitrogen starvation of the NADP+-isocitrate dehydrogenase from the cyanobacterium Phormidium laminosum (1999), Biochim. Biophys. Acta, 1431, 87-96.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | substrate inhibition | Phormidium laminosum |  |
| 5'-ADP | - |
Phormidium laminosum | |
| ATP | - |
Phormidium laminosum | |
| NADPH | - |
Phormidium laminosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
D,L-isocitrate | Mn2+-dependent reaction | Phormidium laminosum | |
| 0.024 | - |
Mn2+ | Mn2+-dependent reaction | Phormidium laminosum | |
| 0.032 | - |
NADP+ | Mg2+-dependent reaction | Phormidium laminosum | |
| 0.05 | - |
NADP+ | Mn2+-dependent reaction | Phormidium laminosum | |
| 0.07 | - |
D,L-isocitrate | Mg2+-dependent reaction | Phormidium laminosum | |
| 0.159 | - |
Mg2+ | Mg2+-dependent reaction | Phormidium laminosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | absolute requirement for divalent cations | Phormidium laminosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
alpha2, 2 * 53000, SDS-PAGE | Phormidium laminosum |
| 107000 | - |
gel filtration | Phormidium laminosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Phormidium laminosum | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phormidium laminosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phormidium laminosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 500 | - |
- |
Phormidium laminosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Phormidium laminosum | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 53000, SDS-PAGE | Phormidium laminosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Phormidium laminosum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | - |
Phormidium laminosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
fully stable | Phormidium laminosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Phormidium laminosum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Phormidium laminosum | |
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