Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Thermoleophilum minutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
D,L-isocitrate | - |
Thermoleophilum minutum | |
0.048 | - |
NADP+ | - |
Thermoleophilum minutum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for divalent cations | Thermoleophilum minutum | |
Mn2+ | absolute requirement for divalent cations | Thermoleophilum minutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
alpha2, 2 * 30500, SDS-PAGE | Thermoleophilum minutum |
60000 | - |
PAGE and gel filtration | Thermoleophilum minutum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Thermoleophilum minutum | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Thermoleophilum minutum YS-4 | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoleophilum minutum | - |
YS-4 | - |
Thermoleophilum minutum YS-4 | - |
YS-4 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermoleophilum minutum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3884 | - |
- |
Thermoleophilum minutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Thermoleophilum minutum | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Thermoleophilum minutum YS-4 | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 30500, SDS-PAGE | Thermoleophilum minutum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermoleophilum minutum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
after 1 h incubation retains 57% of initial activity | Thermoleophilum minutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Thermoleophilum minutum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermoleophilum minutum |