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Literature summary for 1.1.1.42 extracted from

  • Yoon, J.J.; Hattori, T.; Shimada, M.
    Purification and characterization of NADP-linked isocitrate dehydrogenase from the copper-tolerant wood-rotting basidiomycete Fomitopsis palustris (2003), Biosci. Biotechnol. Biochem., 67, 114-120.
    View publication on PubMed

General Stability

General Stability Organism
Mg2+ stabilizes the enzyme during purification Fomitopsis palustris

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 92% inhibition at 5 mM Fomitopsis palustris
ADP 15% inhibition at 2 mM Fomitopsis palustris
ATP 10% inhibition at 2 mM Fomitopsis palustris
Ca2+ in presence of 5 mM Mg2+ 6% of maximal activity is remaining Fomitopsis palustris
Cd2+ complete inhibition at 5 mM in presence of 5 mM Mg2+ Fomitopsis palustris
cis-aconitate 35% inhibition at 5 mM Fomitopsis palustris
citrate 30% inhibition at 1 mM and 5 mM Fomitopsis palustris
Co2+ 92% inhibition in presence of 5 mM Mg2+ Fomitopsis palustris
Cu2+ enzyme is completely inhibited at 5 mM in presence of 5 mM Mg2+, organism is a copper-tolerant basidiomycete Fomitopsis palustris
glyoxylate 18% inhibition at 5 mM, mixed type inhibition together with oxaloacetate, completely at 5 mM each Fomitopsis palustris
Itaconate 18% inhibition at 5 mM Fomitopsis palustris
additional information AMP is a no inhibitor, no inhibition by glutamate, pyruvate and succinate Fomitopsis palustris
oxalate 18% inhibition at 5 mM Fomitopsis palustris
oxaloacetate 41% inhibition at 5 mM, mixed type inhibition together with glyoxylate, completely at 5 mM each Fomitopsis palustris
Zn2+ complete inhibition at 5 mM in presence of 5 mM Mg2+ Fomitopsis palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
NADP+ pH 9.0, 30°C Fomitopsis palustris
0.0127
-
isocitrate pH 9.0, 30°C Fomitopsis palustris
0.0239
-
Mg2+ pH 9.0, 30°C Fomitopsis palustris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Fomitopsis palustris 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Mg2+ by 25% Fomitopsis palustris
Mg2+ required for activity Fomitopsis palustris
Mn2+ can substitute for Mg2+ by 90% Fomitopsis palustris
additional information K+ cannot substitute for Mg2+ Fomitopsis palustris
Na+ can substitute for Mg2+ by 20% Fomitopsis palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, SDS-PAGE Fomitopsis palustris
115000
-
gel filtration Fomitopsis palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Fomitopsis palustris key enzyme of the tricarboxylic acid cycle 2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Fomitopsis palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
672fold to homogeneity Fomitopsis palustris

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Fomitopsis palustris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75
-
purified enzyme Fomitopsis palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NADP+
-
Fomitopsis palustris 2-oxoglutarate + CO2 + NADPH
-
?
isocitrate + NADP+ key enzyme of the tricarboxylic acid cycle Fomitopsis palustris 2-oxoglutarate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Fomitopsis palustris

Synonyms

Synonyms Comment Organism
NADP-dependent isocitrate dehydrogenase
-
Fomitopsis palustris
NADP-IDH
-
Fomitopsis palustris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Fomitopsis palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Fomitopsis palustris

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Fomitopsis palustris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.127
-
2-oxoglutarate pH 9.0, 30°C Fomitopsis palustris