General Stability | Organism |
---|---|
Mg2+ stabilizes the enzyme during purification | Fomitopsis palustris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 92% inhibition at 5 mM | Fomitopsis palustris | |
ADP | 15% inhibition at 2 mM | Fomitopsis palustris | |
ATP | 10% inhibition at 2 mM | Fomitopsis palustris | |
Ca2+ | in presence of 5 mM Mg2+ 6% of maximal activity is remaining | Fomitopsis palustris | |
Cd2+ | complete inhibition at 5 mM in presence of 5 mM Mg2+ | Fomitopsis palustris | |
cis-aconitate | 35% inhibition at 5 mM | Fomitopsis palustris | |
citrate | 30% inhibition at 1 mM and 5 mM | Fomitopsis palustris | |
Co2+ | 92% inhibition in presence of 5 mM Mg2+ | Fomitopsis palustris | |
Cu2+ | enzyme is completely inhibited at 5 mM in presence of 5 mM Mg2+, organism is a copper-tolerant basidiomycete | Fomitopsis palustris | |
glyoxylate | 18% inhibition at 5 mM, mixed type inhibition together with oxaloacetate, completely at 5 mM each | Fomitopsis palustris | |
Itaconate | 18% inhibition at 5 mM | Fomitopsis palustris | |
additional information | AMP is a no inhibitor, no inhibition by glutamate, pyruvate and succinate | Fomitopsis palustris | |
oxalate | 18% inhibition at 5 mM | Fomitopsis palustris | |
oxaloacetate | 41% inhibition at 5 mM, mixed type inhibition together with glyoxylate, completely at 5 mM each | Fomitopsis palustris | |
Zn2+ | complete inhibition at 5 mM in presence of 5 mM Mg2+ | Fomitopsis palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
NADP+ | pH 9.0, 30°C | Fomitopsis palustris | |
0.0127 | - |
isocitrate | pH 9.0, 30°C | Fomitopsis palustris | |
0.0239 | - |
Mg2+ | pH 9.0, 30°C | Fomitopsis palustris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Fomitopsis palustris | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute for Mg2+ by 25% | Fomitopsis palustris | |
Mg2+ | required for activity | Fomitopsis palustris | |
Mn2+ | can substitute for Mg2+ by 90% | Fomitopsis palustris | |
additional information | K+ cannot substitute for Mg2+ | Fomitopsis palustris | |
Na+ | can substitute for Mg2+ by 20% | Fomitopsis palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Fomitopsis palustris |
115000 | - |
gel filtration | Fomitopsis palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Fomitopsis palustris | key enzyme of the tricarboxylic acid cycle | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fomitopsis palustris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
672fold to homogeneity | Fomitopsis palustris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Fomitopsis palustris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
75 | - |
purified enzyme | Fomitopsis palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Fomitopsis palustris | 2-oxoglutarate + CO2 + NADPH | - |
? | |
isocitrate + NADP+ | key enzyme of the tricarboxylic acid cycle | Fomitopsis palustris | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Fomitopsis palustris |
Synonyms | Comment | Organism |
---|---|---|
NADP-dependent isocitrate dehydrogenase | - |
Fomitopsis palustris |
NADP-IDH | - |
Fomitopsis palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Fomitopsis palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Fomitopsis palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Fomitopsis palustris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.127 | - |
2-oxoglutarate | pH 9.0, 30°C | Fomitopsis palustris |