Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.42 extracted from

  • Zhang, B.; Wang, B.; Wang, P.; Cao, Z.; Huang, E.; Hao, J.; Dean, A.M.; Zhu, G.
    Enzymatic characterization of a monomeric isocitrate dehydrogenase from Streptomyces lividans TK54 (2009), Biochimie, 91, 1405-1410.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Streptomyces lividans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 4.94% relative activity at 2 mM Streptomyces lividans
Co2+ 46.47% relative activity at 2 mM Streptomyces lividans
Cu2+ complete inhibition at 2 mM Streptomyces lividans
EDTA complete inhibition at 2 mM Streptomyces lividans
K+ 3.53% relative activity at 2 mM Streptomyces lividans
Na+ 4.67% relative activity at 2 mM Streptomyces lividans
Zn2+ 1.85% relative activity at 2 mM Streptomyces lividans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00242
-
NADP+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ Streptomyces lividans
0.00278
-
NADP+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ Streptomyces lividans
8.49
-
NAD+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ Streptomyces lividans
18.6
-
NAD+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ Streptomyces lividans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the recombinant IDH displays a 85000fold greater specificity for NADP+ than NAD+ with Mg2+ Streptomyces lividans
Mn2+ the recombinant IDH displays a 62000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ Streptomyces lividans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
gel filtration Streptomyces lividans
80000
-
SDS-PAGE Streptomyces lividans

Organism

Organism UniProt Comment Textmining
Streptomyces lividans B2ZAA4
-
-
Streptomyces lividans TK54 B2ZAA4
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-isocitrate + NAD+ slow reaction Streptomyces lividans 2-oxoglutarate + CO2 + NADH
-
?
(2R,3S)-isocitrate + NAD+ slow reaction Streptomyces lividans TK54 2-oxoglutarate + CO2 + NADH
-
?
(2R,3S)-isocitrate + NADP+
-
Streptomyces lividans 2-oxoglutarate + CO2 + NADPH
-
?
(2R,3S)-isocitrate + NADP+
-
Streptomyces lividans TK54 2-oxoglutarate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
monomer 1 * 72000, gel filtration Streptomyces lividans

Synonyms

Synonyms Comment Organism
IDH
-
Streptomyces lividans
NADP+-dependent IDH
-
Streptomyces lividans
NADP-IDH
-
Streptomyces lividans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
around 46°C Streptomyces lividans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38 50 about 50% of IDH activity is preserved at 38°C after 20 min of incubation, the enzyme is inactive at 50°C Streptomyces lividans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.37
-
NAD+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ Streptomyces lividans
2.11
-
NAD+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ Streptomyces lividans
23.2
-
NADP+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ Streptomyces lividans
27.6
-
NADP+ in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ Streptomyces lividans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
in the presence of 2 mM Mn2+ Streptomyces lividans
9
-
in the presence of 2 mM Mg2+ Streptomyces lividans

Cofactor

Cofactor Comment Organism Structure
NADP+ the recombinant IDH displays a 62000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+, and a 85000fold greater specificity for NADP+ than NAD+ with Mg2+ Streptomyces lividans