Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.42 extracted from

  • Zera, A.J.; Newman, S.; Berkheim, D.; Black, C.; Klug, L.; Crone, E.
    Purification and characterization of cytoplasmic NADP+-isocitrate dehydrogenase, and amplification of the NADP+-IDH gene from the wing-dimorphic sand field cricket, Gryllus firmus (2011), J. Insect Sci., 11, 53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.42

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Gryllus firmus
0.0023
-
 NADP+ pH 8.0, 28°C Gryllus firmus
0.0147
-
 isocitrate pH 8.0, 28°C Gryllus firmus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Gryllus firmus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Gryllus firmus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46300
-
 x * 46300, SDS-PAGE Gryllus firmus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Gryllus firmus
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Gryllus firmus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by PEG fractionation, affinity and hydrophobic interaction chromatography Gryllus firmus

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Gryllus firmus
-
gut
-
 Gryllus firmus
-
head
-
 Gryllus firmus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
 Gryllus firmus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 46300, SDS-PAGE Gryllus firmus

Synonyms

Synonyms Comment Organism
NADP+-IDH
-
 Gryllus firmus
NADP+-isocitrate dehydrogenase
-
 Gryllus firmus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Gryllus firmus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Gryllus firmus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Gryllus firmus

General Information

General Information Comment Organism
physiological function in the cytoplasm, NADP+-IDH often contributes significantly to the NADPH pool required for reductive fatty acid biosynthesis Gryllus firmus