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Literature summary for 1.1.1.42 extracted from

  • Jin, M.M.; Wang, P.; Li, X.; Zhao, X.Y.; Xu, L.; Song, P.; Zhu, G.P.
    Biochemical characterization of NADP+-dependent isocitrate dehydrogenase from Microcystis aeruginosa PCC7806 (2013), Mol. Biol. Rep., 40, 2995-3002.
    View publication on PubMed
Search for more literature for 1.1.1.42

Cloned(Commentary)

Cloned (Comment) Organism
gene icd, sequence comparisons, expression of His6-tagged in Escherichia coli strain Rosetta (DE3) Microcystis aeruginosa
gene icd, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) Microcystis aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Microcystis aeruginosa
Co2+
-
 Microcystis aeruginosa
Cu2+ complete inhibition Microcystis aeruginosa
K+ slight inhibition Microcystis aeruginosa
Li+ slight inhibition Microcystis aeruginosa
Na+ slight inhibition Microcystis aeruginosa
Ni2+
-
 Microcystis aeruginosa
Rb+ slight inhibition Microcystis aeruginosa
Zn2+ strong inhibition Microcystis aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis Microcystis aeruginosa
0.0322
-
 NADP+ with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
0.0324
-
 NADP+ with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
0.0326
-
 DL-isocitrate with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
0.0326
-
 DL-isocitrate with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
0.0717
-
 NADP+ with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
0.0797
-
 NADP+ with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
0.1243
-
 DL-isocitrate with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
0.1243
-
 DL-isocitrate with Mn2+, pH 7.5, 37°C Microcystis aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates poorly, 2.48% activity compared to Mn2+ Microcystis aeruginosa
Co2+ activates, 10.22% activity compared to Mn2+ Microcystis aeruginosa
K+ activates slightly, 10.18% activity compared to Mn2+ Microcystis aeruginosa
Li+ activates slightly, 12.63% activity compared to Mn2+ Microcystis aeruginosa
Mg2+ can partially substitute for Mn2+ Microcystis aeruginosa
Mg2+ activates, 72.45% activity compared to Mn2+ Microcystis aeruginosa
Mn2+ required Microcystis aeruginosa
Mn2+ activates, required, best divalent cation Microcystis aeruginosa
additional information the enzyme is completely divalent cation dependent. As compared with Mn2+, MaIDH shows about 2.5times and 4times higher affinities (1/Km) to NADP+ and DL-isocitrate with Mg2+, Cu2+ does not activate the enzyme at all Microcystis aeruginosa
Na+ activates slightly, 10.70% activity compared to Mn2+ Microcystis aeruginosa
Ni2+ activates poorly, 1.40% activity compared to Mn2+ Microcystis aeruginosa
Rb+ activates slightly, 20.67% activity compared to Mn2+ Microcystis aeruginosa
Zn2+ activates poorly, 4.23% activity compared to Mn2+ Microcystis aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52600
-
-
 Microcystis aeruginosa
52600
-
 2 * 52600 Microcystis aeruginosa
105000
-
 gel filtration Microcystis aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-isocitrate + NADP+ Microcystis aeruginosa
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
DL-isocitrate + NADP+ Microcystis aeruginosa
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Microcystis aeruginosa
-
-
-
Microcystis aeruginosa A8YGS2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography Microcystis aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NADP+
-
 Microcystis aeruginosa 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
DL-isocitrate + NADP+
-
 Microcystis aeruginosa 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 52600 Microcystis aeruginosa
homodimer 2 * 52600, SDS-PAGE Microcystis aeruginosa

Synonyms

Synonyms Comment Organism
ICD
-
 Microcystis aeruginosa
NADP+-dependent IDH
-
 Microcystis aeruginosa
NADP+-dependent isocitrate dehydrogenase
-
 Microcystis aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 with Mg2+ Microcystis aeruginosa
45
-
 with Mn2+ Microcystis aeruginosa
50
-
 with Mg2+ Microcystis aeruginosa
50
-
 with Mn2+ Microcystis aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity range Microcystis aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified recombinant enzyme, 20 min, stable Microcystis aeruginosa
40
-
 purified recombinant enzyme, 20 min, loss of 50% of enzyme activity, with Mg2+ Microcystis aeruginosa
45
-
 purified recombinant enzyme, 20 min, loss of 50% of enzyme activity, with Mn2+ Microcystis aeruginosa
45
-
 purified enzyme, 20 min, 50% loss of enzyme activity Microcystis aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.29
-
 DL-isocitrate with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
10.92
-
 DL-isocitrate with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
25.36
-
 NADP+ with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
25.36
-
 NADP+ with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
43.21
-
 DL-isocitrate with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
43.21
-
 DL-isocitrate with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
48.88
-
 NADP+ with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
48.88
-
 NADP+ with Mn2+, pH 7.5, 37°C Microcystis aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 with Mn2+ or Mg2+ Microcystis aeruginosa
7.5
-
 the recombinant MaIDH has a broader pH optimum from pH 6.5 to pH 8.5 Microcystis aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 activity range Microcystis aeruginosa

Cofactor

Cofactor Comment Organism Structure
NADP+ purified recombinant MaIDH is completely specific with NADP+, and no NAD+-dependent activity is detectable Microcystis aeruginosa
NADP+ the enzyme is completely NADP+-dependent and no NAD+-linked activity is detectable Microcystis aeruginosa
NADPH
-
 Microcystis aeruginosa

General Information

General Information Comment Organism
physiological function isocitrate dehydrogenase of Microcystis aeruginosa plays important roles in energy and biosynthesis metabolisms and its catalytic product 2-oxoglutarate provides the carbon skeleton for ammonium assimilation and also constitutes a signaling molecule of nitrogen starvation in cyanobacteria Microcystis aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00335
-
 DL-isocitrate with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
0.00348
-
 DL-isocitrate with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
0.00613
-
 NADP+ with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
0.00787
-
 NADP+ with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
335
-
 DL-isocitrate with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
348
-
 DL-isocitrate with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
613
-
 NADP+ with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
787
-
 NADP+ with Mg2+, pH 7.5, 37°C Microcystis aeruginosa