Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain DEK2004 | Psychromonas marina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03353 | - |
isocitrate | at pH 8.0 and 20°C | Psychromonas marina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.67 mM used in assay conditions | Psychromonas marina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Psychromonas marina | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychromonas marina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Psychromonas marina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
136.03 | - |
His-tagged enzyme, at pH 8.0 and 35°C | Psychromonas marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Psychromonas marina | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
additional information | no activity Is detected when 0.12 mM NAD+, instead of NADP+, is added to the reaction mixture | Psychromonas marina | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Psychromonas marina |
monomer | 1 * 80426, calculated from amino acid sequence | Psychromonas marina |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Psychromonas marina |
NADP+-dependent isocitrate dehydrogenase | - |
Psychromonas marina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Psychromonas marina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme loses 74% of the activity after incubation for 10 min at 30°C | Psychromonas marina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102.98 | - |
isocitrate | at pH 8.0 and 20°C | Psychromonas marina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Psychromonas marina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Psychromonas marina |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3086 | - |
isocitrate | at pH 8.0 and 20°C | Psychromonas marina |