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Literature summary for 1.1.1.42 extracted from

  • Hirota, R.; Tsubouchi, K.; Takada, Y.
    NADP+-dependent isocitrate dehydrogenase from a psychrophilic bacterium, Psychromonas marina (2017), Extremophiles, 21, 711-721 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DEK2004 Psychromonas marina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03353
-
isocitrate at pH 8.0 and 20°C Psychromonas marina

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.67 mM used in assay conditions Psychromonas marina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Psychromonas marina
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Psychromonas marina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Psychromonas marina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
136.03
-
His-tagged enzyme, at pH 8.0 and 35°C Psychromonas marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Psychromonas marina 2-oxoglutarate + CO2 + NADPH + H+
-
?
additional information no activity Is detected when 0.12 mM NAD+, instead of NADP+, is added to the reaction mixture Psychromonas marina ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Psychromonas marina
monomer 1 * 80426, calculated from amino acid sequence Psychromonas marina

Synonyms

Synonyms Comment Organism
IDH
-
Psychromonas marina
NADP+-dependent isocitrate dehydrogenase
-
Psychromonas marina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Psychromonas marina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the enzyme loses 74% of the activity after incubation for 10 min at 30°C Psychromonas marina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
102.98
-
isocitrate at pH 8.0 and 20°C Psychromonas marina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Psychromonas marina

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Psychromonas marina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3086
-
isocitrate at pH 8.0 and 20°C Psychromonas marina