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Literature summary for 1.1.1.42 extracted from

  • Tang, X.; Liu, F.; Sheng, X.; Xing, J.; Zhan, W.
    Recombinant NADP-dependent isocitrate dehydrogenase of Edwardsiella tarda induces both Th1 and Th2 type immune responses and evokes protective efficacy against edwardsiellosis (2018), Vaccine, 36, 2337-2345 .
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme is a vaccine candidate against the fish pathogen Edwardsiella tarda infection. The serum of enzyme-vaccinated flounder exhibit the highest bactericidal activity compared with other groups. The recombinant enzyme can enhance the expressions of interferon-gamma, natural killer enhancing factor, interleukin-6, major histocompatibility complex Ialpha, CD4-1 and CD8alpha Edwardsiella tarda

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Edwardsiella tarda

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Edwardsiella tarda 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Edwardsiella tarda
-
2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+ Edwardsiella tarda EIB202
-
2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Edwardsiella tarda D0Z8Y6
-
-
Edwardsiella tarda EIB202 D0Z8Y6
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap Ni-agarose column chromatography Edwardsiella tarda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Edwardsiella tarda 2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+
-
Edwardsiella tarda EIB202 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Edwardsiella tarda
? x * 45836, calculated from amino acid sequence Edwardsiella tarda
? x * 65000, TRX-tagged enzyme, SDS-PAGE Edwardsiella tarda

Synonyms

Synonyms Comment Organism
IDH
-
Edwardsiella tarda
NADP-dependent isocitrate dehydrogenase
-
Edwardsiella tarda

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Edwardsiella tarda

pI Value

Organism Comment pI Value Maximum pI Value
Edwardsiella tarda calculated from amino acid sequence
-
5.2