Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a vaccine candidate against the fish pathogen Edwardsiella tarda infection. The serum of enzyme-vaccinated flounder exhibit the highest bactericidal activity compared with other groups. The recombinant enzyme can enhance the expressions of interferon-gamma, natural killer enhancing factor, interleukin-6, major histocompatibility complex Ialpha, CD4-1 and CD8alpha | Edwardsiella tarda |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Edwardsiella tarda |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Edwardsiella tarda | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Edwardsiella tarda | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | Edwardsiella tarda EIB202 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Edwardsiella tarda | D0Z8Y6 | - |
- |
Edwardsiella tarda EIB202 | D0Z8Y6 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap Ni-agarose column chromatography | Edwardsiella tarda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Edwardsiella tarda | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | - |
Edwardsiella tarda EIB202 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Edwardsiella tarda |
? | x * 45836, calculated from amino acid sequence | Edwardsiella tarda |
? | x * 65000, TRX-tagged enzyme, SDS-PAGE | Edwardsiella tarda |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Edwardsiella tarda |
NADP-dependent isocitrate dehydrogenase | - |
Edwardsiella tarda |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Edwardsiella tarda |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Edwardsiella tarda | calculated from amino acid sequence | - |
5.2 |