Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 1 mM, complete loss of activity | Arthrobacter sp. I-552 | |
Cd2+ | 1 mM, about 30% residual activity | Arthrobacter sp. I-552 | |
Cu2+ | 1 mM, about 30% residual activity | Arthrobacter sp. I-552 | |
additional information | not inhibitory at 1 mM: Mg2+, Ca2+, K+, Na+, iodoacetic acid, EDTA, o-phenanthroline, ferricyanide | Arthrobacter sp. I-552 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
NAD+ | pH 9, 40°C | Arthrobacter sp. I-552 | |
14 | - |
levoglucosan | pH 9, 40°C | Arthrobacter sp. I-552 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
290000 | - |
gel filtration | Arthrobacter sp. I-552 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
levoglucosan + NAD+ | Arthrobacter sp. I-552 | - |
3-dehydrolevoglucosan + NADH + H+ | - |
? | |
additional information | Arthrobacter sp. I-552 | the reaction follows a sequential mechanism. Catalytic activity is specific for levoglucosan and NAD+. Poor substrates: ribose and glucose | ? | - |
- |
|
sorbose + NAD+ | Arthrobacter sp. I-552 | - |
? + NADH + H+ | - |
? | |
xylose + NAD+ | Arthrobacter sp. I-552 | - |
? + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. I-552 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. I-552 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
levoglucosan + NAD+ | - |
Arthrobacter sp. I-552 | 3-dehydrolevoglucosan + NADH + H+ | - |
? | |
additional information | the reaction follows a sequential mechanism. Catalytic activity is specific for levoglucosan and NAD+. Poor substrates: ribose and glucose | Arthrobacter sp. I-552 | ? | - |
- |
|
additional information | the reaction follows a sequential mechanism. Catalytic activity is specific for levoglucosan and NAD+. Poor or no substrates: ribose and glucose, mannose, galactose, fructose, fucose, rhamnose, galactosan, sedoheptulosan | Arthrobacter sp. I-552 | ? | - |
- |
|
sorbose + NAD+ | - |
Arthrobacter sp. I-552 | ? + NADH + H+ | - |
? | |
xylose + NAD+ | - |
Arthrobacter sp. I-552 | ? + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 28000 plus x * 21000, SDS-PAGE | Arthrobacter sp. I-552 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Arthrobacter sp. I-552 |
50 | - |
optimum temperature | Arthrobacter sp. I-552 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
333 | - |
levoglucosan | pH 9, 40°C | Arthrobacter sp. I-552 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Arthrobacter sp. I-552 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arthrobacter sp. I-552 |