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Literature summary for 1.1.1.425 extracted from

  • Sugiura, M.; Nakahara, M.; Yamada, C.; Arakawa, T.; Kitaoka, M.; Fushinobu, S.
    Identification, functional characterization, and crystal structure determination of bacterial levoglucosan dehydrogenase (2018), J. Biol. Chem., 293, 17375-17386 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.425

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudarthrobacter phenanthrenivorans

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of LgdH in the apo and complex forms with NADH, NADH plus levoglucosan, and NADH plus L-sorbose. LgdH has a typical fold of Gfo/Idh/MocA family proteins. The active site recognizes the levoglucosan molecule with six hydrogen bonds, and the C3 atom of levoglucosan is closely located to the C4 atom of NADH nicotinamide Pseudarthrobacter phenanthrenivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 gel filtration Pseudarthrobacter phenanthrenivorans

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter phenanthrenivorans F0M433
-
-
Pseudarthrobacter phenanthrenivorans DSM 18606 F0M433
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.65
-
 substrate 1,5-anhydro-D-glucitol, pH 8.5, 40°C Pseudarthrobacter phenanthrenivorans
0.73
-
 substrate L-sorbose, pH 8.5, 40°C Pseudarthrobacter phenanthrenivorans
17.8
-
 substrate levosan, pH 8.5, 40°C Pseudarthrobacter phenanthrenivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydrolevoglucosan + NADH + H+
-
 Pseudarthrobacter phenanthrenivorans levoglucosan + NAD+
-
 r
3-dehydrolevoglucosan + NADH + H+
-
 Pseudarthrobacter phenanthrenivorans DSM 18606 levoglucosan + NAD+
-
 r
levoglucosan + NAD+
-
 Pseudarthrobacter phenanthrenivorans 3-dehydrolevoglucosan + NADH + H+
-
 r
levoglucosan + NAD+
-
 Pseudarthrobacter phenanthrenivorans DSM 18606 3-dehydrolevoglucosan + NADH + H+
-
 r
additional information about 4% of the activity with levoglucosan with L-sorbose and 1,5-anhydro-D-glucitol Pseudarthrobacter phenanthrenivorans ?
-
-
additional information about 4% of the activity with levoglucosan with L-sorbose and 1,5-anhydro-D-glucitol Pseudarthrobacter phenanthrenivorans DSM 18606 ?
-
-

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE, 4 * 44280, calculated from sequence Pseudarthrobacter phenanthrenivorans

Synonyms

Synonyms Comment Organism
Asphe3_10730
-
 Pseudarthrobacter phenanthrenivorans
LgdH
-
 Pseudarthrobacter phenanthrenivorans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudarthrobacter phenanthrenivorans
NADH
-
 Pseudarthrobacter phenanthrenivorans