Literature summary for 1.1.1.430 extracted from

Nidetzky, B.; Klimacek, M.; Mayr, P.
Transient-state and steady-state kinetic studies of the mechanism of NADH-dependent aldehyde reduction catalyzed by xylose reductase from the yeast Candida tenuis (2001), Biochemistry, 40, 10371-10381.

Search for more literature for 1.1.1.430

Inhibitors

Inhibitors	Comment	Organism	Structure
NAD+	-	Yamadazyma tenuis
NADH	-	Yamadazyma tenuis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	NADH	pH 7.0, 25°C	Yamadazyma tenuis
0.027	-	NAD+	pH 7.0, 25°C	Yamadazyma tenuis
78	-	D-xylose	pH 7.0, 25°C	Yamadazyma tenuis
209	-	xylitol	pH 7.0, 25°C	Yamadazyma tenuis

Organism

Organism	UniProt	Comment	Textmining
Yamadazyma tenuis	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
xylitol + NAD+ = D-xylose + NADH + H+	chemical mechanism of carbonyl reduction by xylose reductase in which transfer of hydride ion is a partially rate-limiting step and precedes the proton-transfer step	Yamadazyma tenuis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-xylose + NADH + H+	aldehyde reduction is favoured	Yamadazyma tenuis	xylitol + NAD+	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Yamadazyma tenuis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.92	-	NAD+	pH 7.0, 25°C	Yamadazyma tenuis
0.92	-	xylitol	pH 7.0, 25°C	Yamadazyma tenuis
14.2	-	NADH	pH 7.0, 25°C	Yamadazyma tenuis
14.2	-	D-xylose	pH 7.0, 25°C	Yamadazyma tenuis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Yamadazyma tenuis

Cofactor

Cofactor	Comment	Organism	Structure
NADH	transient-state and steady-state kinetic studies of the mechanism of NADH-dependent aldehyde reduction	Yamadazyma tenuis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.016	-	NADH	pH 7.0, 25°C	Yamadazyma tenuis
0.195	-	NAD+	pH 7.0, 25°C	Yamadazyma tenuis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0044	-	xylitol	pH 7.0, 25°C	Yamadazyma tenuis
0.18	-	D-xylose	pH 7.0, 25°C	Yamadazyma tenuis
34.1	-	NAD+	pH 7.0, 25°C	Yamadazyma tenuis
946.7	-	NADH	pH 7.0, 25°C	Yamadazyma tenuis

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Release 2023.1 (January 2023)