KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
NADPH | pH 7.0, 30°C | Rhizomucor pusillus | |
0.35 | - |
NADH | pH 7.0, 30°C | Rhizomucor pusillus | |
28.1 | - |
D-xylose | pH 7.0, 30°C | Rhizomucor pusillus | |
36.3 | - |
D-ribose | pH 7.0, 30°C | Rhizomucor pusillus | |
41.7 | - |
L-arabinose | pH 7.0, 30°C | Rhizomucor pusillus | |
72.2 | - |
D-galactose | pH 7.0, 30°C | Rhizomucor pusillus | |
177 | - |
D-glucose | pH 7.0, 30°C | Rhizomucor pusillus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
- |
Rhizomucor pusillus |
59000 | - |
gel filtration | Rhizomucor pusillus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor pusillus | I7HD84 | - |
- |
Rhizomucor pusillus NBRC 4578 | I7HD84 | - |
- |
Purification (Comment) | Organism |
---|---|
from mycelium | Rhizomucor pusillus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Rhizomucor pusillus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NADH + H+ | 53% of the activity with D-xylose | Rhizomucor pusillus | ? + NAD+ | - |
? | |
D-galactose + NADH + H+ | 53% of the activity with D-xylose | Rhizomucor pusillus NBRC 4578 | ? + NAD+ | - |
? | |
D-glucose + NADPH + H+ | 38% of the activity with D-xylose | Rhizomucor pusillus | ? + NADP+ | - |
? | |
D-glucose + NADPH + H+ | 38% of the activity with D-xylose | Rhizomucor pusillus NBRC 4578 | ? + NADP+ | - |
? | |
D-ribose + NADPH + H+ | 84% of the activity with D-xylose | Rhizomucor pusillus | ? + NADP+ | - |
? | |
D-ribose + NADPH + H+ | 84% of the activity with D-xylose | Rhizomucor pusillus NBRC 4578 | ? + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Rhizomucor pusillus | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Rhizomucor pusillus NBRC 4578 | xylitol + NADP+ | - |
? | |
L-arabinose + NADPH + H+ | 79% of the activity with D-xylose | Rhizomucor pusillus | L-arabinitol + NADP+ | - |
? | |
L-arabinose + NADPH + H+ | 79% of the activity with D-xylose | Rhizomucor pusillus NBRC 4578 | L-arabinitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36724, calculated, 2 * 38000, SDS-PAGE | Rhizomucor pusillus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.3 | - |
D-xylose | pH 7.0, 30°C | Rhizomucor pusillus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rhizomucor pusillus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 2.4% of the activity with NADPH | Rhizomucor pusillus | |
NADPH | - |
Rhizomucor pusillus |
Organism | Comment | Expression |
---|---|---|
Rhizomucor pusillus | transcription of the xyl1 gene is marked in the presence of D-xylose and L-arabinose, but is week in the presence of D-glucose | additional information |