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Literature summary for 1.1.1.431 extracted from
- Efficient biosynthesis of xylitol from xylose by coexpression of xylose reductase and glucose dehydrogenase in Escherichia coli (2019), Appl. Biochem. Biotechnol., 187, 1143-1157 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | efficient biosynthesis of xylitol from xylose by coexpression of xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum, the latter is used for cofactor regeneration, in Escherichia coli strain BL21(DE3)/pCDFDuet-1-XR-GDH, from Escherichia coli strains BL21(DE3)/pET28b(+)-XR and BL21(DE3)/pET28b(+)-GDH. The xylitol yield of the coupled system is maximal at pH 8.0 and 30°C, and at a unit ratio of GDH to XR concentration of 4:1, indicating that the regeneration of coenzyme had a significant effect on xylitol synthesis. Method optimization, overview | Rhizopus arrhizus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylitol + NADP+ | Rhizopus arrhizus | - |
D-xylose + NADPH + H+ | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | W5RZ21 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylitol + NADP+ | - |
Rhizopus arrhizus | D-xylose + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, recombinant enzyme, SDS-PAGE | Rhizopus arrhizus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XYL1 | - |
Rhizopus arrhizus |
| xylose reductase | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rhizopus arrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rhizopus arrhizus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rhizopus arrhizus | |
| NADPH | - |
Rhizopus arrhizus | |
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Release 2023.1 (January 2023)
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