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Literature summary for 1.1.1.44 extracted from

  • Veronese, F.M.; Boccu, E.; Fontana, A.; Benassi, C.A.; Scoffone, E.
    Isolation and some properties of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus (1974), Biochim. Biophys. Acta, 334, 31-44.
No PubMed abstract available
Search for more literature for 1.1.1.44

Application

Application Comment Organism
synthesis thermostability may lead to some practical applications Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Cyberlindnera jadinii
5,5'-dithiobis(2-nitrobenzoic acid) Ellman's reagent Geobacillus stearothermophilus
Hg2+
-
 Geobacillus stearothermophilus
p-chloromercuribenzoate
-
 Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 NADP+ 60°C Geobacillus stearothermophilus
0.014
-
 6-phosphogluconate 60°C or 65°C Geobacillus stearothermophilus
0.015
-
 NADP+ 65°C Geobacillus stearothermophilus
0.016
-
 6-phosphogluconate 30°C Geobacillus stearothermophilus
0.017
-
 NADP+ 30°C Geobacillus stearothermophilus
0.02
-
 6-phosphogluconate 43°C Geobacillus stearothermophilus
0.021
-
 NADP+ 45°C Geobacillus stearothermophilus
0.022
-
 6-phosphogluconate 45°C Geobacillus stearothermophilus
0.025
-
 NADP+ 43°C Geobacillus stearothermophilus
0.55
-
 2-deoxy-6-phosphogluconate
-
 Cyberlindnera jadinii
1
-
 NAD+ 43°C Cyberlindnera jadinii
3.5
-
 NAD+ 30°C Cyberlindnera jadinii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates the enzyme at high concentrations Geobacillus stearothermophilus
Mg2+
-
 Cyberlindnera jadinii
Mg2+ activates the enzyme at low concentrations 0.01-0.04 M Geobacillus stearothermophilus
NaCl activates the enzyme at high concentrations Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 51000, SDS-PAGE Geobacillus stearothermophilus
101000
-
-
 Cyberlindnera jadinii
101000
-
 sucrose density gradient centrifugation Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Homo sapiens
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Rattus norvegicus
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Neurospora crassa
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus NCA 1503
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Geobacillus stearothermophilus
-
 NCA 1503
-
Geobacillus stearothermophilus NCA 1503
-
 NCA 1503
-
Homo sapiens
-
 human
-
Neurospora crassa
-
-
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87
-
-
 Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
4°C, stable for several days, as an (NH4)2SO4 suspension stable for several months without any loss of enzyme activity Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADH
-
 ?
6-phospho-D-gluconate + NADP+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Homo sapiens D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Rattus norvegicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Geobacillus stearothermophilus NCA 1503 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Cyberlindnera jadinii
dimer
-
 Homo sapiens
dimer
-
 Rattus norvegicus
dimer
-
 Neurospora crassa
dimer 2 * 51000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable at 60°C Geobacillus stearothermophilus
70
-
 denaturation half-time of 80 min Geobacillus stearothermophilus
80
-
 denaturation half-time of 3 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cyberlindnera jadinii
8
-
-
 Homo sapiens
8
-
-
 Rattus norvegicus
8
-
-
 Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Cyberlindnera jadinii
NAD+
-
 Rattus norvegicus
NAD+ some activity with NAD+ Geobacillus stearothermophilus
NADP+
-
 Cyberlindnera jadinii
NADP+
-
 Rattus norvegicus
NADP+
-
 Geobacillus stearothermophilus
NADP+
-
 Neurospora crassa