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Literature summary for 1.1.1.47 extracted from

  • Hilt, W.; Pfleidere, G.; Fortnagel, P.
    Glucose dehydrogenase from Bacillus subtilis expressed in Escherichia coli I: purification, characterization and comparison with glucose dehydrogenase from Bacillus megaterium (1991), Biochim. Biophys. Acta, 1076, 298-304.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NAD+ pH 8.0, 25°C Bacillus subtilis
8.7
-
 beta-D-glucose pH 8.0, 25°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sepharose, Blue Fractogel Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
0°C, 67 mM KH2PO4, pH 6.5, 0.5 M NaCl, stable for a few months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+
-
 Bacillus subtilis D-glucono-1,5-lactone + NADH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 shifting the pH from 6.5 to 9.0 leads to inactivation, reactivation to 100% activity by shifting pH back to 6.5, protein concentration higher than 0.1 mg/ml Bacillus subtilis
9
-
 inactivation above, Gluc-DH-S Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis