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Literature summary for 1.1.1.47 extracted from

  • Ding, H.; Gao, F.; Yu, Y.; Chen, B.
    Biochemical and computational insights on a novel acid-resistant and thermal-stable glucose 1-dehydrogenase (2017), Int. J. Mol. Sci., 18, 1198 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.47

Application

Application Comment Organism
biotechnology the robust stability of the enzyme makes it an attractive participant for cofactor regeneration on practical applications, especially for the catalysis implemented in acidic pH and high temperature Bacillus sp. (in: Bacteria)
diagnostics the enzyme can be a potential diagnostic reagent for blood glucose measurement Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
heterogeneously expressed in Escherichia coli BL21 (DE3) Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 NAD+ pH 8.0, 25°C Bacillus sp. (in: Bacteria)
0.404
-
 NADP+ pH 8.0, 25°C Bacillus sp. (in: Bacteria)
17.126
-
 D-glucose pH 8.0, 25°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A0A068FPP9
-
-
Bacillus sp. (in: Bacteria) ZJ A0A068FPP9
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
194
-
 pH 8.0, 25°C, D-glucose + NAD+ Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactose + NAD+ 6.8% of the activity as compared to D-glucose Bacillus sp. (in: Bacteria) D-galactono-1,5-lactone + NADH
-
 ?
beta-D-galactose + NAD+ 6.8% of the activity as compared to D-glucose Bacillus sp. (in: Bacteria) ZJ D-galactono-1,5-lactone + NADH
-
 ?
D-glucose + NAD+ the enzyme prefers NAD+ rather than NADP+ Bacillus sp. (in: Bacteria) D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NAD+ the enzyme prefers NAD+ rather than NADP+ Bacillus sp. (in: Bacteria) ZJ D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NADP+ the enzyme prefers NAD+ rather than NADP+ Bacillus sp. (in: Bacteria) D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose + NADP+ the enzyme prefers NAD+ rather than NADP+ Bacillus sp. (in: Bacteria) ZJ D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-maltose + NAD+ 10% of the activity as compared to D-glucose Bacillus sp. (in: Bacteria) ?
-
 ?
D-maltose + NAD+ 10% of the activity as compared to D-glucose Bacillus sp. (in: Bacteria) ZJ ?
-
 ?
D-xylose + NADP+ 6.1% of the activity as compared to D-glucose Bacillus sp. (in: Bacteria) D-xylono-1,5-lactone + NADPH + H+
-
 ?
D-xylose + NADP+ 6.1% of the activity as compared to D-glucose Bacillus sp. (in: Bacteria) ZJ D-xylono-1,5-lactone + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 30000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
bzgdh
-
 Bacillus sp. (in: Bacteria)
GDH
-
 Bacillus sp. (in: Bacteria)
NAD(P)-dependent glucose 1-dehydrogenase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 25°C: about 60% of maximal activity, 55°C: about 50% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable at temperatures below 50°C Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73.96
-
 NADP+ pH 8.0, 25°C Bacillus sp. (in: Bacteria)
84.52
-
 NAD+ pH 8.0, 25°C Bacillus sp. (in: Bacteria)
87.84
-
 D-glucose pH 8.0, 25°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 glycine-NaOH buffer Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8.5 1 h incubation, the enzyme maintains around 80% of its initial activity Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme prefers NAD+ rather than NADP+ Bacillus sp. (in: Bacteria)
NADP+ the enzyme prefers NAD+ rather than NADP+ Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) calculated from sequence
-
 5.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.13
-
 D-glucose pH 8.0, 25°C Bacillus sp. (in: Bacteria)
182.98
-
 NADP+ pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1166.29
-
 NAD+ pH 8.0, 25°C Bacillus sp. (in: Bacteria)