Organism | UniProt | Comment | Textmining |
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Saccharomyces cerevisiae | - |
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Purification (Comment) | Organism |
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continuous counter-current purification of glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae cells by liquid-liquid extraction using reverse micelles. A biocompatible reverse micellar system consisting of 0.05 M soybean lecithin (zwitterionic surfactants) in isooctane with hexanol is employed to study the influence of different flow-rates on G6PD purification. The results show that the reverse micellar system is able to remove proteins (impurities) from the cell-free. The enzyme recovery yield varies from 32% to 115% and G6PD purification factor from 1.0 to 2.2, under flow-rates ranging from 1.6:1.6 mL/min to 6.0:6.0 ml/min for both phases (micellar and aqueous phases). The steady hold-up values demonstrate that the mass transfer capability of this extraction apparatus is practically constant. The continuous counter-current purification system employed proves to be useful for purifying glucose-6-phosphate dehydrogenase and for maintaining the enzyme activity | Saccharomyces cerevisiae |