Literature summary for 1.1.1.49 extracted from

Boonyuen, U.; Chamchoy, K.; Swangsri, T.; Saralamba, N.; Day, N.P.; Imwong, M.
Detailed functional analysis of two clinical glucose-6-phosphate dehydrogenase (G6PD) variants, G6PDViangchan and G6PDViangchan+Mahidol: Decreased stability and catalytic efficiency contribute to the clinical phenotype (2016), Mol. Genet. Metab., 118, 84-91.

Search for more literature for 1.1.1.49

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	the kcat value of the mutant enzymes is markedly reduced, resulting in a 10 and 18fold reduction in catalytic efficiency for NADP+ catalysis for mutant G6PDViangchan and mutant G6PDViangchan+Mahidol, respectively	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Trypsin	after 2 h of trypsin digestion in the presence of 0.01 mM NADP+ the enzyme retains about 57% of its original activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0072	-	NADP+	at pH 8.0 and 37°C	Homo sapiens
0.0478	-	D-glucose 6-phosphate	at pH 8.0 and 37°C	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	Homo sapiens	-	6-phospho-D-glucono-1,5-lactone + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P11413	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap QXL column chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0443	-	crude extract, at pH 8.0 and 37°C	Homo sapiens
0.2282	-	purified enzyme, at pH 8.0 and 37°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	-	Homo sapiens	6-phospho-D-glucono-1,5-lactone + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
G6PD	-	Homo sapiens
glucose-6-phosphate dehydrogenase	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
47	-	the half-life temperature is 47°C	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
247	-	NADP+	at pH 8.0 and 37°C	Homo sapiens
247	-	D-glucose 6-phosphate	at pH 8.0 and 37°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5200	-	D-glucose 6-phosphate	at pH 8.0 and 37°C	Homo sapiens
34300	-	NADP+	at pH 8.0 and 37°C	Homo sapiens

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Release 2023.1 (January 2023)