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Literature summary for 1.1.1.50 extracted from

  • Seitz, H.U.; Gaertner, D.E.
    Enzymes in cardenolide-accumulating shoot cultures of Digitalis purpurea (1994), Plant Cell Tissue Organ Cult., 38, 337-344.
No PubMed abstract available
Search for more literature for 1.1.1.50

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.235
-
 5beta-Pregnan-3alpha-ol-20-one cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
0.4
-
 NADH cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
0.91
-
 NADPH cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
1.33
-
 NADPH microsomal NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, 97% in the cytosol Digitalis purpurea 5829
-
microsome low activity of NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity Digitalis purpurea
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-pregnan-3,20-dione + NAD(P)H + H+ Digitalis purpurea 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors 5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
 ?
5beta-pregnan-3,20-dione + NAD(P)H Digitalis purpurea 3alpha-hydroxysteroid-5beta-oxidoreductase activity 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 r

Organism

Organism UniProt Comment Textmining
Digitalis purpurea
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
shoot culture Digitalis purpurea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
 Digitalis purpurea 5beta-pregnan-3,20-dione + NADPH
-
 r
5alpha-pregnan-3,20-dione + NAD(P)H + H+ 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors Digitalis purpurea 5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
 ?
5beta-pregnan-3,20-dione + NAD(P)H 3alpha-hydroxysteroid-5beta-oxidoreductase activity Digitalis purpurea 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 r
5beta-pregnane-3,20-dione + NADH + H+
-
 Digitalis purpurea 5beta-pregnan-3alpha-ol-20-one + NAD+
-
 r

Synonyms

Synonyms Comment Organism
3alpha-hydroxysteroid-5beta-oxidoreductase
-
 Digitalis purpurea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Digitalis purpurea
NADH cytosolic 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity Digitalis purpurea
NADPH NADPH-linked microsomal 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase shows very low activity only with NADPH Digitalis purpurea