Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic modeling of an enzymatic cycling reaction | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | ordered bi bi kinetic reaction mechanism with nucleotide cofactor binding first | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Pseudomonas sp. |
3alpha-hydroxysteroid dehydrogenase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | kinetic model involving cofactor-enzyme complex formation kinetics | Pseudomonas sp. | |
NAD+ | - |
Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
thio-NAD+ | - |
Pseudomonas sp. |