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Literature summary for 1.1.1.50 extracted from
- Examination of the differences in structure-function of human and rat 3alpha-hydroxysteroid dehydrogenase (2003), Chem. Biol. Interact., 143-144, 383-392.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a potential drug target | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isozymes AKR1C1-4 in Escherichia coli | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isozyme AKR1C2 in ternary complex with NADP+ and ursodeoxycholate, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement | Homo sapiens |
| isozyme AKR1C9 in ternary complex with NADP+ and ursodeoxycholate, X-ray diffraction structure determination and analysis at 2.8 A resolution | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | cofactor binding kinetics | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | Homo sapiens | isozyme AKR1C4 | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r |  |
| 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | Rattus norvegicus | isozyme AKR1C9 | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
| additional information | Rattus norvegicus | enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | ? | - |
? | |
| additional information | Homo sapiens | physiological roles of the isozymes AKR1C1-4, overview, enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
4 isozymes AKR1C1-4 | - |
| Rattus norvegicus | - |
isozyme AKR1C9 | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | steroid binding pocket structure of isozyme AKR1C2 | Homo sapiens | |
| a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | steroid binding pocket structure of isozyme AKR1C9 | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | isozyme AKR1C4 | Homo sapiens | - |
| prostate | isozyme AKR1C4 | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | isozyme AKR1C4 | Homo sapiens | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
| 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | isozyme AKR1C9 | Rattus norvegicus | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
| 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | preferred substrate for isozyme AKR1C4, while isozymes AKR1C1-3 show only low activity | Homo sapiens | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
| additional information | enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | Rattus norvegicus | ? | - |
? | |
| additional information | physiological roles of the isozymes AKR1C1-4, overview, enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | Homo sapiens | ? | - |
? | |
| additional information | isozyme is positional- and stereospecific for the 3alpha-hydroxy steroid dehydrogenase reaction | Rattus norvegicus | ? | - |
? | |
| additional information | isozymes display 3alpha-, 17beta-, and 20alpha-hydroxysteroid dehydrogenase activities to varying degrees, isozyme AKR1C4 shows the highest 3alpha-hydroxysteroid dehydrogenase activity of all isozymes, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | isozyme AKR1C2 shows an (alphabeta)8-barrel structure | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3alpha-hydroxysteroid dehydrogenase | - |
Homo sapiens |
| 3alpha-hydroxysteroid dehydrogenase | - |
Rattus norvegicus |
| AKR1C1-4 | - |
Homo sapiens |
| AKR1C9 | - |
Rattus norvegicus |
| HSD | - |
Homo sapiens |
| HSD | - |
Rattus norvegicus |
| More | enzyme belongs to the aldo-keto reductase superfamily | Homo sapiens |
| More | enzyme belongs to the aldo-keto reductase superfamily | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
5alpha-dihydrotestosterone | isozyme AKR1C2 | Homo sapiens | |
| 1 | - |
5alpha-dihydrotestosterone | isozyme AKR1C9 | Rattus norvegicus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | binding and release mechanism | Homo sapiens | |
| NADP+ | binding and release mechanism | Rattus norvegicus | |
| NADPH | binding and release mechanism | Homo sapiens | |
| NADPH | binding and release mechanism | Rattus norvegicus | |
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