Literature summary for 1.1.1.50 extracted from

Kataoka, S.; Nakamura, S.; Ohkubo, T.; Ueda, S.; Uchiyama, S.; Kobayashi, Y.; Oda, M.
Crystallization and preliminary X-ray analysis of the complex of NADH and 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 (2006), Acta Crystallogr. Sect. F, 62, 569-571.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in complex with NADH, hanging drop vapour diffusion method and microseeding, 0.002 ml of protein solution with 0.3 mM 3alpha-HSD and 0.4 mM NADH in 10 mM TrisHCl pH 8.0, are mixed with 0.002 ml reservoir solution optimally containing 0.1 M TrisHCl, pH 9.0, 0.14 M sodium chloride and 1.4 M ammonium sulfate, 4°C, X-ray diffraction structure determination and analysis at 1.8 A resolution	Pseudomonas sp.

Crystallization (Comment)

Organism

purified recombinant enzyme in complex with NADH, hanging drop vapour diffusion method and microseeding, 0.002 ml of protein solution with 0.3 mM 3alpha-HSD and 0.4 mM NADH in 10 mM TrisHCl pH 8.0, are mixed with 0.002 ml reservoir solution optimally containing 0.1 M TrisHCl, pH 9.0, 0.14 M sodium chloride and 1.4 M ammonium sulfate, 4°C, X-ray diffraction structure determination and analysis at 1.8 A resolution

Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	2 * 26000, recombinant enzyme	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme catalyzes the reversible interconversion of hydroxyl and oxo groups at position 3 of the steroid nucleus	Pseudomonas sp.	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 26000, recombinant enzyme	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
3alpha-HSD	-	Pseudomonas sp.
3alpha-hydroxysteroid dehydrogenase	-	Pseudomonas sp.
More	the enzyme belongs to the short-chain dehydrogenase/reductase, SDR, superfamily	Pseudomonas sp.
NAD(P)+-3alpha-hydroxysteroid dehydrogenase	-	Pseudomonas sp.

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Pseudomonas sp.