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Literature summary for 1.1.1.50 extracted from

  • Jin, Y.; Penning, T.M.
    Multiple steps determine the overall rate of the reduction of 5alpha-dihydrotestosterone catalyzed by human type 3 3alpha-hydroxysteroid dehydrogenase: implications for the elimination of androgens (2006), Biochemistry, 45, 13054-13063.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain C41(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information transient single and multiple turnover kinetics, stopped flow kinetics, isotope kinetics, ligand binding analysis, kinetics with deuterium-substituted cofactor and binary complex of enzyme and cofactor, overview Homo sapiens
0.0029
-
5alpha-dihydrotestosterone pH 7.0, 25°C, recombinant enzyme Homo sapiens
0.0031
-
3alpha-androstanediol pH 7.0, 25°C, recombinant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-dihydrotestosterone + NADPH + H+ Homo sapiens inactivation of the potent androgen in human prostate 5alpha-androstan-3alpha,17beta-diol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain C41(DE3) in a successive chromatographic procedure, to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-acenaphthenol + NADPH
-
Homo sapiens ?
-
?
5alpha-dihydrotestosterone + NADPH + H+ inactivation of the potent androgen in human prostate Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NADP+
-
r
5alpha-dihydrotestosterone + NADPH + H+ i.e. DHT Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NADP+
-
r

Synonyms

Synonyms Comment Organism
AKR1C2
-
Homo sapiens
type 3 3alpha-hydroxysteroid dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
3alpha-androstanediol pH 7.0, 25°C, recombinant enzyme Homo sapiens
0.033
-
5alpha-dihydrotestosterone pH 7.0, 25°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH comparison of NADPH with deuterium-substituted NADPD, overview Homo sapiens