Application | Comment | Organism |
---|---|---|
biotechnology | transfection of cells with plasmids encoding a 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein. The Del1 deposition domain immobilizes the enzyme in the extracellular matrix without interfering with its enzymatic activity. Extracellular matrix conditioned by cells transfected with 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion significantly suppresses the growth of otherwise untreated LNCaP cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in COS-7 cells | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, 3alpha-hydroxysteroid dehydrogenase, x * 65000, 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein, SDS-PAGE | Homo sapiens |
65000 | - |
x * 43000, 3alpha-hydroxysteroid dehydrogenase, x * 65000, 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | Q91WR5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
LNCaP cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,17beta-androstene-3,17-diol + NAD(P)+ | - |
Mus musculus | dihydrotestosterone + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, 3alpha-hydroxysteroid dehydrogenase, x * 65000, 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
3 alphaHD | - |
Mus musculus |
3alpha-hydroxysteroid dehydrogenase | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Mus musculus |