Cloned (Comment) | Organism |
---|---|
sequence comparison and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pseudomonas aeruginosa | |
CO2 | - |
Pseudomonas aeruginosa | |
Cu2+ | 67% inhibition at 0.5 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | activates | Pseudomonas aeruginosa | |
K+ | activates | Pseudomonas aeruginosa | |
Mn2+ | activates | Pseudomonas aeruginosa | |
additional information | no effect by 0.5 mM of Mg2+, Ni2+, and Al3+ | Pseudomonas aeruginosa | |
Na+ | activates | Pseudomonas aeruginosa | |
Zn2+ | activates 1.75fold at 0.5 mM | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3alpha-hydroxysteroid + NAD+ | Pseudomonas aeruginosa | - |
a 3-oxosteroid + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I1X3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3alpha-hydroxysteroid + NAD+ | - |
Pseudomonas aeruginosa | a 3-oxosteroid + NADH + H+ | - |
r | |
cholate + NAD+ | - |
Pseudomonas aeruginosa | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Pseudomonas aeruginosa |
3alpha-hydroxysteroid dehydrogenase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | optimum at 30°C, over 60% of maximal activity in the range of 25-45°C, with the temperature increasing from 30°C to 45°C, the enzyme activity gradually decreases to 77.55% of the optimum whereas with the temperature decreasing from 30°C to 15°C, the enzyme activity rapidly drops to 39.4% of the optimum | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His-tagged enzyme, stable up to | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, profile overview | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa | |
NADH | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | 3alpha-hydroxysteroid dehydrogenase catalyzes the oxidation of the 3-hydroxyl group of steroids. The enzymatic conversion is a critical step in the enzymatic assay of urinary sulfated bile acids | Pseudomonas aeruginosa |