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Literature summary for 1.1.1.52 extracted from
- Reversible enzymatic oxidation of bile acids (1955), Arch. Biochem. Biophys., 56, 554-555.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | SH-groups are required for activity | Escherichia sp. |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia sp. | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3alpha-hydroxy-5beta-cholanate + NAD+ | Escherichia sp. | - |
3-oxo-5beta-cholanate + NADH | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia sp. | - |
tentative identification: E. freundii | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3alpha-hydroxy-5beta-cholanate + NAD+ | - |
Escherichia sp. | 3-oxo-5beta-cholanate + NADH | - |
r | |
| 3alpha-hydroxy-5beta-cholanate + NAD+ | i.e. lithocholic acid | Escherichia sp. | 3-oxo-5beta-cholanate + NADH | - |
r | |
| 3alpha-hydroxy-bis-norcholanic acid + NAD+ | oxidation at the same rate as hydroxycholanic acid | Escherichia sp. | 3-oxo-bis-norcholanic acid + NADH | - |
r | |
| 3alpha-hydroxy-norcholanic acid + NAD+ | oxidation at the same rate as hydroxycholanic acid | Escherichia sp. | 3-oxo-norcholanic acid + NADH | - |
r | |
| cholic acid + NAD+ | oxidation at the same rate as hydroxycholanic acid | Escherichia sp. | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| dehydrocholic acid + NADH | i.e. 3,7,12-trioxocholan-24-oic acid | Escherichia sp. | 3-hydroxy-7,12-dihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
r | |
| deoxycholic acid + NAD+ | oxidation at the same rate as hydroxycholanic acid | Escherichia sp. | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
r | |
| additional information | no substrates: androsterone and tetrahydrocortisone, methylation of carboxyl group or reduction to alcohol inactivates | Escherichia sp. | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
assay at | Escherichia sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
oxidation, assay at | Escherichia sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
oxidation proceeds at alkaline and reduction favorably at neutral pH-values | Escherichia sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NADP+ cannot replace NAD+ | Escherichia sp. | |
| NADH | - |
Escherichia sp. | |
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