Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Sus scrofa | |
Cu2+ | - |
Sus scrofa | |
Fe2+ | - |
Sus scrofa | |
Hg2+ | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
progesterone | reduction | Sus scrofa | |
0.00016 | - |
5alpha-pregnane-3,20-dione | reduction | Sus scrofa | |
0.00018 | - |
5alpha-androstan-17beta-ol-3-one | reduction | Sus scrofa | |
0.00032 | - |
5alpha-Androstan-3alpha-ol-17-one | oxidation | Sus scrofa | |
0.00094 | - |
17alpha-hydroxyprogesterone | reduction | Sus scrofa | |
7.2 | - |
beta-NADPH | - |
Sus scrofa | |
14.8 | - |
beta-NADP+ | - |
Sus scrofa | |
88.2 | - |
beta-NAD+ | - |
Sus scrofa | |
126.1 | - |
alpha-NADH | - |
Sus scrofa | |
294.8 | - |
beta-NADH | - |
Sus scrofa | |
328 | - |
alpha-NADPH | - |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
purified enzyme, NADPH, progesterone | Sus scrofa |
0.019 | - |
purified enzyme, NADPH, 5beta-androstan-3alpha,17beta-diol oxidation | Sus scrofa |
0.023 | - |
purified enzyme, NADPH, dehydroisoandrosterone | Sus scrofa |
0.025 | - |
purified enzyme, NADPH, 5alpha-androstan-3alpha-ol-17-one oxidation | Sus scrofa |
0.046 | - |
purified enzyme, NADPH, 5alpha-androstan-17beta-ol-3-one | Sus scrofa |
0.051 | - |
purified enzyme, NADPH, 5alpha-pregnane-3,20-dione | Sus scrofa |
0.052 | - |
purified enzyme, NADPH, 5alpha-androstan-3alpha,17beta-diol oxidation | Sus scrofa |
0.091 | - |
purified enzyme, NADPH, 17alpha-methyl-5alpha-androstan-17beta-ol-3-one | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Sus scrofa | 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ | - |
? | |
5alpha-androstan-17beta-ol-3-one + NAD(P)H + H+ | i.e. 5alpha-dihydrotestosterone | Sus scrofa | 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | ratio 4:3 | ? | |
5alpha-androstan-3alpha-ol-17-one + alpha-NAD+ | - |
Sus scrofa | 5alpha-dihydrotestosterone + alpha-NADH + H+ | - |
r | |
5alpha-androstan-3alpha-ol-17-one + beta-NAD+ | - |
Sus scrofa | 5alpha-dihydrotestosterone + beta-NADH + H+ | - |
r | |
5alpha-androstane-3,17-dione + NADPH | - |
Sus scrofa | ? | - |
? | |
5alpha-dihydrotestosterone + alpha-NADH + H+ | - |
Sus scrofa | 5alpha-androstan-3alpha-ol-17-one + alpha-NAD+ | - |
r | |
5alpha-dihydrotestosterone + alpha-NADPH + H+ | - |
Sus scrofa | 5alpha-androstan-3alpha-ol-17-one + alpha-NADP+ | - |
r | |
5alpha-dihydrotestosterone + beta-NADH + H+ | - |
Sus scrofa | 5alpha-androstan-3alpha-ol-17-one + beta-NAD+ | - |
r | |
5alpha-pregnane-3,20-dione + NADPH | - |
Sus scrofa | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of 5alpha-androstan-17beta-ol-3-one with beta-NADPH | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa | |
NADH | - |
Sus scrofa | |
NADP+ | - |
Sus scrofa | |
NADPH | hydrogen transfer is stereospecific | Sus scrofa |