Characterization of a Pseudomonas putida allylic alcohol dehydrogenase induced by growth on 2-methyl-3-buten-2-ol

Malone, V.F.; Chastian, A.J.; Ohlsson, J.T.; Poneleit, L.S.; Nemecek-Marshall, M.; Fall, R.; Appl. Environ. Microbiol. 65, 2622-2630 (1999)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

3-methyl-2-buten-1-al

product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay

Pseudomonas putida

dithiothreitol

-

Pseudomonas putida

EDTA

only at concentration of 100 mM 50% reduction

Pseudomonas putida

N-ethylmaleimide

-

Pseudomonas putida

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.007

-

benzyl alcohol

-

Pseudomonas putida

0.007

-

3-chloro-2-buten-1-ol

-

Pseudomonas putida

0.011

-

3-aminobenzyl alcohol

-

Pseudomonas putida

0.012

-

(R)-(+)-perillyl alcohol

-

Pseudomonas putida

0.036

-

cinnamyl alcohol

-

Pseudomonas putida

0.04

-

3-methyl-2-buten-1-ol

-

Pseudomonas putida

0.044

-

2-Buten-1-ol

-

Pseudomonas putida

0.064

-

NAD+

with 3-methyl-2-buten-1-ol

Pseudomonas putida

0.118

-

NAD+

with benzyl alcohol

Pseudomonas putida

0.27

-

3-methyl-1-butanol

-

Pseudomonas putida

1.1

-

1-butanol

-

Pseudomonas putida

1.7

-

3-methyl-3-buten-1-ol

-

Pseudomonas putida

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

3-methyl-2-buten-1-ol + NAD+

Pseudomonas putida

can use substrate as sole carbon source

3-methyl-2-buten-1-al + NADH

-

Pseudomonas putida

?

3-methyl-2-buten-1-ol + NAD+

Pseudomonas putida MB-1

can use substrate as sole carbon source

3-methyl-2-buten-1-al + NADH

-

Pseudomonas putida MB-1

?

Reaction

Commentary

Organism

Reaction ID

allyl alcohol + NADP+ = acrolein + NADPH + H+

3-methyl-2-buten-1-ol-dependent inducible activity

Pseudomonas putida

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

(R)-(+)-perillyl alcohol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

1-butanol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

2-buten-1-ol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

2-buten-2-ol + NADPH

-

287114

Pseudomonas putida

but-2-enal

-

-

-

?

2-buten-2-ol + NADPH

-

287114

Pseudomonas putida MB-1

but-2-enal

-

-

-

?

3-aminobenzyl alcohol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

3-chloro-2-buten-1-ol + NADP+

-

287114

Pseudomonas putida

3-chloro-2-butenaldehyde + NADPH

-

-

-

?

3-chloro-2-buten-1-ol + NADP+

-

287114

Pseudomonas putida MB-1

3-chloro-2-butenaldehyde + NADPH

-

-

-

?

3-methyl-1-butanol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

3-methyl-2-buten-1-ol + NAD+

-

287114

Pseudomonas putida

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida

?

3-methyl-2-buten-1-ol + NAD+

can use substrate as sole carbon source

287114

Pseudomonas putida

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida

?

3-methyl-2-buten-1-ol + NAD+

-

287114

Pseudomonas putida MB-1

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida MB-1

?

3-methyl-2-buten-1-ol + NAD+

can use substrate as sole carbon source

287114

Pseudomonas putida MB-1

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida MB-1

?

3-methyl-3-buten-1-ol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

benzyl alcohol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

cinnamyl alcohol + NADP+

-

287114

Pseudomonas putida

cinnamyl aldehyde + NADPH

-

-

-

?

cinnamyl alcohol + NADP+

-

287114

Pseudomonas putida MB-1

cinnamyl aldehyde + NADPH

-

-

-

?

Cofactor

Commentary

Organism

Structure

NAD+

exhibits less than 5% of the activity with NADP+

Pseudomonas putida

Cofactor

Commentary

Organism

Structure

NAD+

exhibits less than 5% of the activity with NADP+

Pseudomonas putida

Inhibitors

Commentary

Organism

Structure

3-methyl-2-buten-1-al

product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay

Pseudomonas putida

dithiothreitol

-

Pseudomonas putida

EDTA

only at concentration of 100 mM 50% reduction

Pseudomonas putida

N-ethylmaleimide

-

Pseudomonas putida

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.007

-

benzyl alcohol

-

Pseudomonas putida

0.007

-

3-chloro-2-buten-1-ol

-

Pseudomonas putida

0.011

-

3-aminobenzyl alcohol

-

Pseudomonas putida

0.012

-

(R)-(+)-perillyl alcohol

-

Pseudomonas putida

0.036

-

cinnamyl alcohol

-

Pseudomonas putida

0.04

-

3-methyl-2-buten-1-ol

-

Pseudomonas putida

0.044

-

2-Buten-1-ol

-

Pseudomonas putida

0.064

-

NAD+

with 3-methyl-2-buten-1-ol

Pseudomonas putida

0.118

-

NAD+

with benzyl alcohol

Pseudomonas putida

0.27

-

3-methyl-1-butanol

-

Pseudomonas putida

1.1

-

1-butanol

-

Pseudomonas putida

1.7

-

3-methyl-3-buten-1-ol

-

Pseudomonas putida

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

3-methyl-2-buten-1-ol + NAD+

Pseudomonas putida

can use substrate as sole carbon source

3-methyl-2-buten-1-al + NADH

-

Pseudomonas putida

?

3-methyl-2-buten-1-ol + NAD+

Pseudomonas putida MB-1

can use substrate as sole carbon source

3-methyl-2-buten-1-al + NADH

-

Pseudomonas putida MB-1

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

(R)-(+)-perillyl alcohol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

1-butanol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

2-buten-1-ol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

2-buten-2-ol + NADPH

-

287114

Pseudomonas putida

but-2-enal

-

-

-

?

2-buten-2-ol + NADPH

-

287114

Pseudomonas putida MB-1

but-2-enal

-

-

-

?

3-aminobenzyl alcohol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

3-chloro-2-buten-1-ol + NADP+

-

287114

Pseudomonas putida

3-chloro-2-butenaldehyde + NADPH

-

-

-

?

3-chloro-2-buten-1-ol + NADP+

-

287114

Pseudomonas putida MB-1

3-chloro-2-butenaldehyde + NADPH

-

-

-

?

3-methyl-1-butanol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

3-methyl-2-buten-1-ol + NAD+

-

287114

Pseudomonas putida

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida

?

3-methyl-2-buten-1-ol + NAD+

can use substrate as sole carbon source

287114

Pseudomonas putida

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida

?

3-methyl-2-buten-1-ol + NAD+

-

287114

Pseudomonas putida MB-1

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida MB-1

?

3-methyl-2-buten-1-ol + NAD+

can use substrate as sole carbon source

287114

Pseudomonas putida MB-1

3-methyl-2-buten-1-al + NADH

-

287114

Pseudomonas putida MB-1

?

3-methyl-3-buten-1-ol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

benzyl alcohol + NADP+

-

287114

Pseudomonas putida

?

-

-

-

r

cinnamyl alcohol + NADP+

-

287114

Pseudomonas putida

cinnamyl aldehyde + NADPH

-

-

-

?

cinnamyl alcohol + NADP+

-

287114

Pseudomonas putida MB-1

cinnamyl aldehyde + NADPH

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

724009

Zhu

Coproduction of acetaldehyde a ...

Saccharomyces cerevisiae

Appl. Environ. Microbiol.

77

6441-6450

2011

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

725090

Corrales Escobosa

Fusarium oxysporum Adh1 has du ...

Fusarium oxysporum

Fungal Genet. Biol.

48

886-895

2011

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

685505

Matsushima

An enone reductase from Nicoti ...

Nicotiana tabacum

Bioorg. Chem.

36

23-28

2008

-

-

1

-

-

-

-

3

-

-

4

-

-

3

-

-

1

-

-

-

-

-

5

3

2

1

-

-

2

1

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

3

-

-

4

-

-

-

-

1

-

-

-

-

5

3

1

-

-

2

1

-

-

-

-

-

-

-

-

-

287113

Hirata

A 38 kDa allylic alcohol dehyd ...

Nicotiana tabacum

Phytochemistry

55

297-303

2000

-

-

1

-

-

-

-

-

-

-

2

1

-

3

-

-

1

-

-

-

1

-

4

1

-

-

-

-

-

2

-

-

2

-

-

-

-

-

1

2

-

-

-

-

-

-

-

-

-

2

1

-

-

-

1

-

-

1

-

4

1

-

-

-

-

2

-

-

-

-

-

-

-

-

-

287114

Malone

Characterization of a Pseudomo ...

Pseudomonas putida, Pseudomonas putida MB-1

Appl. Environ. Microbiol.

65

2622-2630

1999

-

-

-

-

-

-

4

12

-

1

2

2

-

2

-

-

1

1

-

-

12

1

17

1

-

-

-

-

-

2

-

1

1

-

-

-

-

-

-

1

-

-

-

-

4

-

12

-

1

2

2

-

-

-

1

-

-

12

1

17

1

-

-

-

-

2

-

1

-

-

-

-

-

-

-

287112

Otsuka

-

Triphosphopyridine nucleotide- ...

Escherichia coli

J. Gen. Appl. Microbiol.

4

211-215

1958

-

-

-

-

-

-

5

2

-

1

-

1

-

1

-

-

1

-

-

-

1

-

4

-

-

-

-

-

-

1

-

-

2

-

-

-

-

-

-

2

-

-

-

-

5

-

2

-

1

-

1

-

-

-

1

-

-

1

-

4

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-