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Literature summary for 1.1.1.56 extracted from
- Purification and characterization of a ribitol dehydrogenase from Enterobacter agglomerans strain 221e (1995), J. Ferment. Bioeng., 79, 496-498.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition | Pantoea agglomerans |  |
| Cu2+ | 10-20% inhibition | Pantoea agglomerans | |
| Hg2+ | 10-20% inhibition | Pantoea agglomerans | |
| Ni2+ | 10-20% inhibition | Pantoea agglomerans | |
| Zn2+ | 10-20% inhibition | Pantoea agglomerans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.36 | - |
NAD+ | pH 9, 30°C | Pantoea agglomerans | |
| 32.2 | - |
ribitol | pH 9, 30°C | Pantoea agglomerans | |
| 84.2 | - |
L-arabitol | pH 9, 30°C | Pantoea agglomerans | |
| 107 | - |
allitol | pH 9, 30°C | Pantoea agglomerans | |
| 178 | - |
L-sorbitol | pH 9, 30°C | Pantoea agglomerans | |
| 233 | - |
L-mannitol | pH 9, 30°C | Pantoea agglomerans | |
| 273 | - |
xylitol | pH 9, 30°C | Pantoea agglomerans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | slight increase | Pantoea agglomerans | |
| Sn2+ | slight increase | Pantoea agglomerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
4 * 25000, SDS-PAGE | Pantoea agglomerans |
| 92000 | - |
gel filtration | Pantoea agglomerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pantoea agglomerans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pantoea agglomerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 144 | - |
- |
Pantoea agglomerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allitol + NAD+ | - |
Pantoea agglomerans | D-psicose + NADH | - |
r | |
| D-sorbitol + NAD+ | - |
Pantoea agglomerans | L-sorbose + NADH + H+ | - |
? | |
| erythritol + NAD+ | - |
Pantoea agglomerans | ? + NADH | - |
? | |
| L-arabitol + NAD+ | - |
Pantoea agglomerans | L-xylulose + NADH + H+ | - |
? | |
| L-mannitol + NAD+ | - |
Pantoea agglomerans | ? | - |
r | |
| L-sorbitol + NAD+ | - |
Pantoea agglomerans | ? | - |
r | |
| L-talitol + NAD+ | - |
Pantoea agglomerans | L-tagatose + NADH | - |
r | |
| ribitol + NAD+ | - |
Pantoea agglomerans | D-ribulose + NADH | - |
? | |
| xylitol + NAD+ | - |
Pantoea agglomerans | D-xylulose + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 25000, SDS-PAGE | Pantoea agglomerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | alcohol oxidation | Pantoea agglomerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 11 | - |
Pantoea agglomerans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pantoea agglomerans | |
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