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Literature summary for 1.1.1.56 extracted from

  • Tran, K.N.; Pham, N.; Jang, S.H.; Lee, C.
    Purification and characterization of a novel medium-chain ribitol dehydrogenase from a lichen-associated bacterium Sphingomonas sp. (2020), PLoS ONE, 15, e0235718 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.56

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 NAD+ pH 8.0, 25°C Sphingomonas sp.
8.5
-
 D-ribitol pH 8.0, 25°C Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
 gel filtration Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribitol + NAD+ the catalytic amino acid residues of SpRDH consist of Cys37, His65, Glu66, and Glu157 Sphingomonas sp. D-ribulose + NADH + H+
-
 ?
D-xylitol + NAD+ at 66% of the activity with ribitol Sphingomonas sp. ? + NADH + H+
-
 ?
additional information no dehydrogenation activity for D-mannitol, D-sorbitol, D-galactitol, myo-inositol, or glycerol Sphingomonas sp. ?
-
-

Subunits

Subunits Comment Organism
trimer 3 * 39000, SDS-PAGE Sphingomonas sp.

Synonyms

Synonyms Comment Organism
ribitol dehydrogenase
-
 Sphingomonas sp.
SpRDH
-
 Sphingomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Sphingomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 55% of maximal activity, 70°C: about 70% of maximal activity Sphingomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 40 2.5 h, stable Sphingomonas sp.
50
-
 3 h, 30% loss of activity Sphingomonas sp.
60
-
 20 min, complete loss of activity Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
 D-ribitol pH 8.0, 25°C Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in phosphate buffer Sphingomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 50% of maximal activity, pH 9.5: about 50% of maximal activity, in phosphate buffer Sphingomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sphingomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5
-
 D-ribitol pH 8.0, 25°C Sphingomonas sp.