Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Priestia megaterium | |
2,2'-bipyridyl | - |
Priestia megaterium | |
3,4-dimercaptotoluol | - |
Priestia megaterium | |
8-hydroxyquinoline | - |
Priestia megaterium | |
diphosphate | - |
Priestia megaterium | |
EDTA | - |
Priestia megaterium | |
Imidazol | - |
Priestia megaterium | |
pyridin-2,6-dicarboxylic acid | - |
Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
NAD+ | pH 9.0, 25°C | Priestia megaterium | |
1.4 | - |
glycerol | pH 9.0, 25°C | Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Priestia megaterium | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1.6 mol Zn2+ bound per subunit | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Priestia megaterium |
156000 | 160000 | density gradient centrifugation, gel filtration | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Priestia megaterium | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58.1 | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | - |
Priestia megaterium | hydroxyacetone + NADH | - |
? | |
erythrite + NAD+ | - |
Priestia megaterium | ? | - |
? | |
glycerol + NAD+ | - |
Priestia megaterium | dihydroxyacetone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, SDS-PAGE | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min stable | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADH | - |
Priestia megaterium |